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biomill8358

无虫 (小有名气)

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虫号: 689651
注册: 2009-01-08

[交流] 【分享】由MII卵母细胞高效诱导体细胞重编程

High-efficiency somatic reprogramming induced by intactMII oocytes
由MII卵母细胞高效诱导体细胞重编程
HuiYang1, LinyuShi1, ShenghuaZhang1, JiangweiLin1, Jing Jiang1, JinsongLi1
1Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,
Chinese Academy of Sciences, 320 YueyangRoad, Shanghai 200031, China
Cell Research (2010) 20:1034-1042
摘要：Somatic nuclei can be reprogrammed into a pluripotent state by nuclear transfer, cell fusion and expression of transcription factors. However, these reprogramming processes are very inefficient, which has greatly hindered efforts to elucidate the underlying molecular mechanisms. Here, we report a new reprogramming strategy that combines the advantages of all three reprogramming methodologies into one process. We injected nuclei from cumulus cells into intact MII oocytes. Following activation, 80% of the reconstructed embryos developed to the blastocyst stage, and tetraploid (4N) embryonic stem (ES) cell lines were generated at a rate of 30% per reconstructed oocyte. We also generated triploid (3N) ES cells after injection of somatic nuclei into activated oocytes. 4N and 3N ES cells expressed pluripotent markers and differentiated into cell types of three embryonic germ layers in vivo. Moreover,all ES cells generated histocompatible, differentiated cells after being engrafted in immunocompetent B6D2F1 mice,showing that ES cells derived from this reprogramming strategy might serve as a source of genetically tailored tissues for transplantation. Thus, we have established a simple and highly efficient reprogramming procedure that provides a system for investigating the molecular mechanisms involved in somatic reprogramming.
1.这篇文章中，作者报道了集三种重编程方法的优点于一体的新的重编程策略。作者将卵丘细胞的细胞核注射到完整的MII卵母细胞中。激活之后，有80%的重组的胚胎发育到了囊胚阶段，并且每一个重组的卵母细胞形成4倍体的ES细胞的几率是30%。
2.作者将体细胞核注射到激活的卵母细胞中后也获得了3倍体的ES细胞。4N和3N的ES细胞都表达多功能标记并且在体外能分化到三层胚胎。
3.所有的ES细胞在移入具有免疫活性的B6D2F1老鼠体内都能产生组织相容性，分化的细胞。这表明用这种方法获得的ES细胞可以用作组织移植的来源。作者为研究体细胞重编程的分子机制提供了一个简单高校的系统。
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