| 查看: 3304 | 回复: 6 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[交流]
【求助/交流】求高手：多肽如何标记FITC？
|
|||
| 我合成的一个九肽想标记FITC，多肽是水溶性的，在DMF中反应没反应成功，MS检测分子量不对。不知有谁做过类似的实验，想请教一下具体的反应条件，最好能给个详细的Protocol，先谢谢了！ |
回复此楼» 猜你喜欢
Wnt-C59：PORCN靶向化学探针，Wnt信号通路阻断机制与科研应用全解析
已经有0人回复
-
Fexaramine：肠道限制性FXR化学探针，结构特征与信号通路研究全解析
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有223人回复
-
NVS-STG2：靶向STING跨膜域的分子胶类激动剂，结构特征与寡聚化机制研究全解析
已经有0人回复
-
DOTA-GPC3肽（CAS 3066439-94-6）：双功能分子探针前体的结构特征与标记应用解析
已经有0人回复
-
科研用 TLR7/8 双激动剂怎么选？现货与质控要点
已经有0人回复
-
SEL120-34A HCl：CDK8/CDK19高选择性激酶抑制剂
已经有0人回复
-
氢氧化钾活化操作
已经有18人回复
-
散金
已经有68人回复
-
生物质超级电容器
已经有0人回复
» 本主题相关商家推荐: (我也要在这里推广)
» 本主题相关价值贴推荐，对您同样有帮助:
- 用碳管负载纳米金做TEM如何制样？
已经有12人回复
- 胍基上的氨基能用FITC标记上吗？
已经有12人回复
- 做壳聚糖的FITC标记，如何测FITC结合含量？
已经有10人回复
- 牛血清蛋白（BSA）的荧光素（FITC）标记步骤及注意事项
已经有12人回复
- 关于FITC的反应
已经有4人回复
- 标记有FITC的多肽分子量为3300左右，能透过用多聚甲醛固定后的细胞吗？
已经有6人回复
- FITC标记蛋白中的Sephadex G25柱
已经有7人回复
- 【求助】FITC荧光检测的文献，要看到纳米粒子在细胞内的分布
已经有18人回复
- 【求助】FITC标记氨基葡萄糖
已经有4人回复
3楼2010-12-24 16:25:53
2楼2010-12-24 16:24:43
★ ★
scelab(金币+2):谢谢提供！:tiger06: 2010-12-24 23:20:48
scelab(金币+2):谢谢提供！:tiger06: 2010-12-24 23:20:48
|
上海生工有进口荧光标记试剂盒卖。 HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine) 蛋白罗丹明标记试剂盒 Description: (5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.(5/6) TAMRA absorbs green visible light at 546nm and emits an orange-red visible light at a maximum emission of 575nm.The NHS ester group provides the simplest and most commonly used group for labeling proteins. The succinimidyl ester group reacts with primary amines in lysine side chains and N-terminal amines forming a stable, covalent amide bond.This kit utilizes Tube-O-REACTOR™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort. The (5/6) TAMRA-SE labeling reagent is also available in our single use OneQuant™ format. Features: Dye preweighed and supplied in single use vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time and no protein loss.application for Labeling of proteins, peptides and nucleic acids with a red fluorescent dye.;Suitable for antibody labeling.Store: -15～-20℃ 编号 包装 价格 产地 786-058 5 Reactions 2,486 GBios HOOK™ Dye Labeling Kit (FITC) 蛋白FITC荧光素标记试剂盒 Description: FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 520nm, respectively, therefore emitting green visible light.This kit utilizes Tube-O-Reactor™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort. Features: Dye preweighed and supplied in single use vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time.application for Labeling of a green fluorescent dye to proteins and peptides. And Suitable for antibody labeling. Store: -15～-20℃ 编号 包装 价格 产地 786-059 5 Reactions 2,486 GBios |
4楼2010-12-24 21:48:43
5楼2010-12-25 19:22:54
28