[交流] 【讨论】在创建钾离子通道蛋白四聚体时出现问题 已有3人参与

在创建钾离子通道蛋白四聚体时出现问题，我按照tutorial操作的过程如下： 在01-BUILD目录下： vmd Extensions ——》Tk Console mol load pdb 1K4C.pdb set all [atomselect top all] $all set segname A $all writepdb KCSA-A.pdb $all delete set sel [atomselect top "all and not name K"] $sel set segname B $sel move {{-1.0 0.0 0.0 310.66} {0.0 -1.0 0.0 310.66} {0.0 0.0 1.0 0.0} {0.0 0.0 0.0 1.0}} $sel writepdb KCSA-B.pdb $sel delete mol delete top mol load pdb 1K4C.pdb set sel [atomselect top "all and not name K"] $sel set segname C $sel move {{0.0 -1.0 0.0 310.66} {1.0 0.0 0.0 0.0} {0.0 0.0 1.0 0.0} {0.0 0.0 0.0 1.0}} $sel writepdb KCSA-C.pdb $sel delete mol delete top mol load pdb 1K4C.pdb set sel [atomselect top "all and not name K"] $sel set segname D $sel move {{0.0 1.0 0.0 0.0} {-1.0 0.0 0.0 310.66} {0.0 0.0 1.0 0.0} {0.0 0.0 0.0 1.0}} $sel writepdb KCSA-D.pdb $sel delete Exit VMD cat KCSA-A.pdb KCSA-B.pdb KCSA-C.pdb KCSA-D.pdb > KCSA.pdb gedit KCSA.pdb，然后 delete the lines: END CRYST1 155.330 155.330 76.270 90.00 90.00 90.00 P 1 1 再存储为KCSA-ALL.pdb 再次在在01-BUILD目录下打开VMD，发现KCSA-ALL.pdb不是四聚体构象 而01-BUILD目录的example-out里的KCSA-ALL.pdb用vmd打开后是四聚体构象： 我查看了我的KCSA-A.pdb  KCSA-B.pdb   KCSA-C.pdb   KCSA-D.pdb 坐标，发现坐标没有重复的啊。然后用VMD依次打开发现正好可以构成一个四聚体的构象，而且和example-out里的KCSA-ALL.pdb一样。这是依次打开后所构成的图请看：

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