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the protocol is following:
1. Use 1¨C2¦Ìl of the DNA fragment.
2. Add 1¦Ìl of Taq DNA Polymerase Reaction 10X Buffer.
3. Add 1¦Ìl of 25mM MgCl2
4. Add dATP to a final concentration of 0.2mM.
5. Add 5 units of Taq DNA Polymerase.
6. Add Nuclease-Free Water to a final volume of 10¦Ìl.
7. Incubate at 70¡ãC for 30 minutes.
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4Â¥2010-03-30 21:48:52
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Originally posted by lxj341401 at 2010-03-30 21:17:32:
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Originally posted by huangdi07 at 2010-03-30 18:25:33:
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