Znn3bq.jpeg
²é¿´: 1631  |  »Ø¸´: 10
µ±Ç°Ö÷ÌâÒѾ­´æµµ¡£

huangdi07

Ìú¸Ëľ³æ (СÓÐÃûÆø)

[½»Á÷] ¡¾ÇóÖú/½»Á÷¡¿taqø ¼ÓA

ÎÒÓÃPFUø×öPCR£¬×îºóÏë¼ÓA£¬ÓõÄÊÇdNTP,Äܱ£Ö¤×îºó¼ÓµÄÊÇAÂ𣿲»ÊǺÜÃ÷°×°¡£¬ÓÐÄÄλ¸ßÊÖÖ¸µãһϡ«¡«Ð»Ð»
»Ø¸´´ËÂ¥
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

lxj341401

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

¡ï ¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
1949stone(½ð±Ò+1): 2010-03-31 08:35
taqø µÄÌØÐÔ¾ÍÊǼÓA,²»¼Ó±ðµÄ.
2Â¥2010-03-30 21:17:32
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yujiaping1

ľ³æ (ÖøÃûдÊÖ)

¡ï ¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
1949stone(½ð±Ò+1): 2010-03-31 08:35
¿Ï¶¨²»ÐС£
ºÃÏñÊÇÔÚ·´Ó¦ÀïÌí¼ÓһЩTaqø¾Í¿ÉÒÔÁË
3Â¥2010-03-30 21:18:37
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

sutao1979

ľ³æ (ÖøÃûдÊÖ)

Сľ³æ10Äêgroupleader

¡ï ¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
1949stone(½ð±Ò+1): 2010-03-31 08:36
the protocol is following:
1. Use 1¨C2¦Ìl of the DNA fragment.
2. Add 1¦Ìl of Taq DNA Polymerase Reaction 10X Buffer.
3. Add 1¦Ìl of 25mM MgCl2
4. Add dATP to a final concentration of 0.2mM.
5. Add 5 units of Taq DNA Polymerase.
6. Add Nuclease-Free Water to a final volume of 10¦Ìl.
7. Incubate at 70¡ãC for 30 minutes.
»áµ±Áè¾ø¶¥£¬Ò»ÀÀÖÚɽС
4Â¥2010-03-30 21:48:52
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

iaminxanadu

ľ³æ (ÕýʽдÊÖ)

¡ï ¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
1949stone(½ð±Ò+1): 2010-03-31 08:36
Ïë×öTA¿Ë¡£¬¾ÍÒªÔÚpfuÀ©Ôöºó»ØÊÕPCR²úÎ֮ºó¼ÓTaq polymerase ÒÔ¼°ÆäËûµÄPCR·´Ó¦³É·Ö£¬»òÕßÒÔdATPÌæ´údNTP£¬37¶ÈÎÂÔ¡°ëСʱ¾Í¿ÉÒÔÁË¡£¡£¡£
5Â¥2010-03-30 23:41:10
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

gyesang

ÈÙÓþ°æÖ÷ (ÖøÃûдÊÖ)

ÓÅÐã°æÖ÷ÓÅÐã°æÖ÷

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
ÒýÓûØÌû:
Originally posted by lxj341401 at 2010-03-30 21:17:32:
taqø µÄÌØÐÔ¾ÍÊǼÓA,²»¼Ó±ðµÄ.

Äã˵µÄ²»×¼È·£¬taq DNA polymerase¼ÓβʱÊÇÓÅÏȼÓA£¬µ«Ã»ÓÐA´æÔÚ£¬Ëû»á¼ÓÆäËûµÄ¼î»ù£¬Õâ¾ÍÊÇÎÒÃÇÓÐʱºòÓÃTaqø¿ÉÒÔÖÆ±¸TÔØÌ壬¾ÍÊǼÓTβ
ѧÊõÎÊÌâÌÖÂÛ×Éѯ·¢Óʼþgyesang@163.com
6Â¥2010-03-30 23:46:35
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

gyesang

ÈÙÓþ°æÖ÷ (ÖøÃûдÊÖ)

ÓÅÐã°æÖ÷ÓÅÐã°æÖ÷

ÒýÓûØÌû:
Originally posted by huangdi07 at 2010-03-30 18:25:33:
ÎÒÓÃPFUø×öPCR£¬×îºóÏë¼ÓA£¬ÓõÄÊÇdNTP,Äܱ£Ö¤×îºó¼ÓµÄÊÇAÂ𣿲»ÊǺÜÃ÷°×°¡£¬ÓÐÄÄλ¸ßÊÖÖ¸µãһϡ«¡«Ð»Ð»

¿ÉÒԵģ¬×îºÃÊǽ«²úÎï´¿»¯Ò»Ï£¬È»ºóÓÃdNTP»òÕßdATP£¬¶¼¿ÉÒԵģ¬ÓÃtaqø°ëСʱ£¬²»ÌᳫÔÚ·´Ó¦ÌåϵÖÐÖ±½Ó¼Ótaq£¬ÒòΪpfu»¹ÊǻὫ¼ÓAβÇеô
ѧÊõÎÊÌâÌÖÂÛ×Éѯ·¢Óʼþgyesang@163.com
7Â¥2010-03-30 23:48:43
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

dfl204

Ìú¸Ëľ³æ (ÖøÃûдÊÖ)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
ÎÒÃÇÒ»°ãÊÇÔÚÌí¼ÓTaq£¬dATPºóÔÚ72¶È30min£¬Ö»Òª²»ÊÇÆ¬¶ÎÌ«³¤£¬×öTA¿Ë¡µÄЧÂʺܸߡ£
8Â¥2010-03-31 20:27:10
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yangjunzju

ľ³æ (ÕýʽдÊÖ)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
TAQøÀ©Ôö²»¾ÍºÃÁËÂï¡£¡£¡£Ä©¶Ë¼ÓA
9Â¥2010-03-31 21:45:13
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

spiderboy

½ð³æ (ÕýʽдÊÖ)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
Â¥Ö÷£¬¼ÓAʵÑéÎÒ×ö¹ýºÜ¶à´ÎÁË¡£ÓÃTAQø£¬Ñ¡ÓÃÕý³£µÄPCR·´Ó¦Ìåϵ¾Í¿ÉÒÔÁË£¬ÓÃdNTP¾Í¿ÉÒÔÁË£¬±Ï¾¹ÊµÑéÊÒ²»Ò»¶¨ÓÐdATP,£¬72¶È°ëСʱÒÔÉÏ£¬È»ºó×ö¹ýÖù´¿»¯Æ¬¶Î¼´¿É£¬²»ÓÃÅܵçÓ¾¡£×îºóÌôÑ¡µ½µÃÑôÐÔ¿Ë¡±ÈÀý»¹ÊÇͦ¸ßµÄ¡£¡£
10Â¥2010-03-31 22:57:03
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ huangdi07 µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] Ò»Ö¾Ô¸»¦9£¬326ÇóÉúÎïѧµ÷¼Á +12 Áõīī 2026-04-13 12/600 2026-04-18 23:31 by ·²¡Çé
[¿¼ÑÐ] »¯Ñ§070300 Çóµ÷¼Á +29 ¹þ¹þ¹þ^_^ 2026-04-12 29/1450 2026-04-18 15:56 by Equinoxhua
[¿¼ÑÐ] 260Çóµ÷¼Á +4 Zyt1314520.. 2026-04-17 5/250 2026-04-18 08:28 by babysonlkd
[¿¼ÑÐ] Ò»Ö¾Ô¸Öпƴó²ÄÁÏÓ뻯¹¤£¬353·Ö»¹Óе÷¼ÁѧУÂð +10 ·ñ¼«Ì©À´2026 2026-04-15 12/600 2026-04-17 17:54 by mapenggao
[¿¼ÑÐ] 295·ÖÇóµ÷¼Á +5 ?ÒªÉϰ¶? 2026-04-17 5/250 2026-04-17 16:51 by fenglj492
[¿¼ÑÐ] 26ҩѧר˶105500Çóµ÷¼Á +6 à¶¹þ¼ÓÓÍ 2026-04-13 7/350 2026-04-16 14:31 by zhouxiaoyu
[¿¼²©] É격×Ô¼ö +3 LinxiaÁÖÏÄ 2026-04-13 3/150 2026-04-16 12:55 by Ä«ºÉ֮¶
[¿¼ÑÐ] 327Çóµ÷¼Á +26 Xxjc1107. 2026-04-13 29/1450 2026-04-16 10:52 by Espannnnnol
[¿¼ÑÐ] Çóµ÷¼ÁÍÆ¼ö +8 СÄô°®Ñ§Ï° 2026-04-14 8/400 2026-04-16 07:22 by ѧԱJpLReM
[¿¼ÑÐ] 085404 22408 309·ÖÇóµ÷¼Á +9 lzmk 2026-04-14 10/500 2026-04-15 20:02 by ѧԱJpLReM
[¿¼ÑÐ] 085801µçÆø×¨Ë¶272Çóµ÷¼Á +19 µçÆøÀî 2026-04-13 21/1050 2026-04-15 13:37 by ºÚ¿Æ¼¼¿óÒµ
[½Ìʦ֮¼Ò] ת³¤Æ¸ÁË +7 ¼òµ¥»¯xn 2026-04-13 7/350 2026-04-14 14:50 by xindong
[¿¼ÑÐ] 105500ҩѧÇóµ÷¼Á +4 x_skys 2026-04-12 4/200 2026-04-14 13:37 by rndfc
[¿¼ÑÐ] 245Çóµ÷¼Á +6 ±ùÌÇéÙ?ÆûË® 2026-04-13 10/500 2026-04-14 10:49 by jyl0317
[¿¼ÑÐ] 085600²ÄÁÏÓ뻯¹¤329·ÖÇóµ÷¼Á +24 Ò¶zilin 2026-04-13 25/1250 2026-04-14 09:20 by ÊÔ¹ÜÆÆÁÑ
[¿¼ÑÐ] ¿¼ÑÐÓ¢Ò»ÊýÒ»338·Ö +9 ³¤½­´óѧ¶«Ð£Çø 2026-04-13 10/500 2026-04-14 00:41 by Íõ¬Bè±
[¿¼ÑÐ] 293Çóµ÷¼Á +16 ÎÒ°®¸ßÊý¸ßÊý°®Î 2026-04-12 18/900 2026-04-13 21:47 by ѧԱJpLReM
[¿¼ÑÐ] 302Çóµ÷¼Á +10 Ò×£¡? 2026-04-13 10/500 2026-04-13 19:04 by lbsjt
[¿¼ÑÐ] 297¹¤¿Æ£¬Çóµ÷¼Á? +13 ºÓÄÏũҵ´óѧ-ÄÜ 2026-04-12 13/650 2026-04-13 14:12 by dingyanbo1
[¿¼ÑÐ] 339Çóµ÷¼Á +4 hanwudada 2026-04-12 4/200 2026-04-13 12:03 by À¶ÔÆË¼Óê
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û