Znn3bq.jpeg
ÉÇÍ·´óѧº£Ñó¿ÆÑ§½ÓÊܵ÷¼Á
²é¿´: 3130  |  »Ø¸´: 5
µ±Ç°Ö÷ÌâÒѾ­´æµµ¡£
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

jlzkchong

гæ (³õÈëÎÄ̳)

[½»Á÷] ¡¾ÇóÖú/½»Á÷¡¿Ë«Ã¸ÇеÄÁ½¸öø×îÊÊ·´Ó³Î¶Ȳ»Ò»ÑùÔõô°ì£¿

Çë½Ì³æÓÑ£¬×î½üÎÒÔÚ×öÖÊÁ£µÄ˫øÇУ¬ÆäÖÐÓõ½Á½×éø£¬BamHI+SalIºÍClaI+SalI·Ö±ð½øÐÐ˫øÇУ¬Ã¸¾ù¹º×ÔTAKARA¹«Ë¾£¬Í¨¹ý²úƷĿ¼²éѯ£¬·¢ÏÖÁ½×éøÖеÄÁ½¸öøµÄ×îÊÊζȶ¼ÊÇÒ»¸ö30¶È£¬Ò»¸ö37¶È£¬ÎÒ¿ªÊ¼Ã»·¢ÏÖÕâ¸öÎÊÌ⣬»ìºÏºóÖ±½Ó·Åµ½37¶ÈøÇУ¬½á¹û·¢ÏÖÖÊÁ£ËƺõÖ»±»µ¥Ã¸ÇÐÁË£¬Ã»ÓÐÇÐÏÂÄ¿µÄСÌõ´ø£¬ÇëÎʸ÷λ³æÓÑÓÐûÓнâ¾öÀàËÆÎÊÌâµÄºÃ·½·¨£¬ÔÚ´Ëл¹ýÁË£¡
»Ø¸´´ËÂ¥
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

tianpingpe

гæ (СÓÐÃûÆø)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
Ñ¡ÔñøÇÐЧÂʶ¼±È½Ï¸ßµÄbuffer£¬ÏÈÔÚ30¶È·´Ó¦2hr£¬È»ºóÔÚ37¶È·´Ó¦£¬Ò»°ã¶¼Ã»ÓÐÌ«´óÎÊÌ⡣Ƭ¶ÎСµÄ»°£¬¾Í¶àøÇм¸¸öÌåϵ£¬ÔÚÒ»¸ö¿×µçÓ¾£¬±£Ö¤Á¿ºÍøÇÐЧÂÊ
4Â¥2009-12-08 09:39:58
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 6 ¸ö»Ø´ð

zhuifeng7000

ÖÁ×ðľ³æ (ÖøÃûдÊÖ)

¡ï
Сľ³æ(½ð±Ò+0.5):¸ø¸öºì°ü£¬Ð»Ð»»ØÌû½»Á÷
1¡¢ÏÈÓÃ30¶ÈµÄøµ¥Ã¸ÇУ¬»ØÊÕ»»Buffer¼ÓºÍøÔÙ37¶ÈøÇС£µ«ÊÇ»¹Òª»ØÊÕ£¬Ã¸ÇеÄÖÊÁ£¿ÉÄÜÓÐËðʧ¡£
2¡¢Ò²¿ÉÒÔ30¶ÈÇÐÒ»¶Îʱ¼äºóÔٷŵ½37¶ÈÇС£ÕâÑù±È½Ïʡʣ¬µ«ÊÇÓÐʱºòø¿ÉÄÜÇеIJ»ÍêÈ«¡£
3¡¢Ò²ÓпÉÄÜÄãµÄÖÊÁ£ÒѾ­ÇпªÁË£¬µ«ÊÇÄãÅܵçÓ¾µÄʱ¼ä½Ï³¤£¬µ¼ÖÂСƬ¶Î¿´²»¼ûÁË£¬ÒòΪСƬ¶Î½áºÏµÄEB¿Ï¶¨ÉÙ£¬Ò»°ã±È½Ï°µ¡£
2Â¥2009-12-07 22:36:55
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

qiang84zhang

Òø³æ (ÕýʽдÊÖ)

½¨ÒéÓÃÂ¥ÉϵĵÚÒ»ÖÖ·½·¨,ÎÒÃǶ¼ÊÇÕâô×öµÄ
3Â¥2009-12-08 09:39:25
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

×î°®»¨Ê«Óê

½ð³æ (ÕýʽдÊÖ)

Çî¹í

ÑÏÓÚÂɼº£¬¿íÒÔ´ýÈË
5Â¥2009-12-08 10:02:10
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] ҩѧÇóµ÷¼Á +11 à¶¹þ¼ÓÓÍ 2026-04-14 13/650 2026-04-14 21:14 by qingfeng258
[¿¼ÑÐ] ²ÄÁÏÏà¹Ø×¨Òµ344Çóµ÷¼ÁË«·Ç¹¤¿ÆÑ§Ð£»ò¿ÎÌâ×é +21 hualkop 2026-04-12 22/1100 2026-04-14 17:44 by lhj2009
[¿¼ÑÐ] 085404 22408 309·ÖÇóµ÷¼Á +7 lzmk 2026-04-14 7/350 2026-04-14 17:02 by lihaoda1994
[¿¼ÑÐ] 297£¬¹¤¿Æµ÷¼Á?ºÓÄÏũҵ´óѧ±¾¿Æ +9 ºÓÄÏũҵ´óѧ-ÄÜ 2026-04-14 9/450 2026-04-14 16:03 by Art1977
[¿¼ÑÐ] 290Çóµ÷¼Á +21 luoziheng 2026-04-10 23/1150 2026-04-14 15:49 by zs92450
[¿¼ÑÐ] 279ѧ˶ʳƷרҵÇóµ÷¼ÁԺУ 20+6 ¹Â¶ÀµÄÀǰ®³ÔÑò 2026-04-12 28/1400 2026-04-14 15:44 by zs92450
[¿¼ÑÐ] »úе¹¤³Ì313·ÖÕÒ¹¤¿Æµ÷¼Á +4 ˫һÁ÷±¾¿Æ»úе 2026-04-08 4/200 2026-04-14 07:32 by Abskk
[¿¼ÑÐ] Çóµ÷¼Á +3 ÎÒ°®¸ßÊý¸ßÊý°®Î 2026-04-12 3/150 2026-04-14 01:00 by Íõ¬Bè±
[¿¼ÑÐ] BÇø0809 £¬ÊýÒ»Ó¢Ò»£¬290 Çóµ÷¼Á +3 ãöΫ1111 2026-04-12 4/200 2026-04-13 20:35 by ѧԱJpLReM
[¿¼ÑÐ] Ò»Ö¾Ô¸2110£¬»¯Ñ§Ñ§Ë¶310·Ö£¬±¾¿ÆÖصãË«·ÇÇóµ÷¼Á +20 ŬÁ¦·Ü¶·112 2026-04-08 20/1000 2026-04-13 14:24 by ÕÅzhihao
[¿¼ÑÐ] Ò»Ö¾Ô¸»ª¹¤085600 331·Ö +7 ÌìÏÂww 2026-04-09 7/350 2026-04-13 09:01 by lhj2009
[¿¼ÑÐ] Ò»Ö¾Ô¸»ªÖÐũ΢ÉúÎ288·Ö£¬ÈýÄêʵÑé¾­Àú +11 ´úfish 2026-04-09 11/550 2026-04-12 10:21 by Hayaay
[¿¼ÑÐ] 085410 273Çóµ÷¼Á +10 X1999 2026-04-09 10/500 2026-04-12 09:24 by ÄæË®³Ë·ç
[¿¼ÑÐ] 296Çóµ÷¼Á +14 Íô£¡£¿£¡ 2026-04-08 15/750 2026-04-11 20:28 by dongdian1
[¿¼ÑÐ] 352 Çóµ÷¼Á +6 yzion 2026-04-11 8/400 2026-04-11 16:24 by Ã÷Ô´ËʱÓÐ
[¿¼ÑÐ] 283Çóµ÷¼Á£¬¹¤¿Æ£¡ +12 ËÕ´òË®7777 2026-04-08 12/600 2026-04-11 10:28 by ÄæË®³Ë·ç
[¿¼ÑÐ] »úеר˶270Çóµ÷¼Á£¬½ÓÊÜ¿çרҵ +12 ÀÏʦ¿´¿´ÎÒ°Éaba 2026-04-09 14/700 2026-04-11 10:21 by laoshidan
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤µ÷¼Á +12 ·ñ¼«Ì©À´2026 2026-04-10 13/650 2026-04-11 00:28 by wangjihu
[¿¼ÑÐ] 265Çóµ÷¼Á +12 ·ç˵ËýÔçÍüÁË 2026-04-10 13/650 2026-04-10 18:56 by chemisry
[¿¼ÑÐ] µ÷¼Á +12 ÔÂ@163.com 2026-04-08 12/600 2026-04-09 14:27 by rl1980
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û