[交流] [求助]一句话翻译

Originally posted by gyesang at 2009-10-15 13:56:
大概意思是，柱子先用10 mM Tris^HCl。。。。这种缓冲液平衡，然后再上样

Originally posted by fykxy_206 at 2009-10-15 16:41:
平衡的意思就是，在你的柱子装好之后，让柱子里面的凝胶或者树脂充分的吸收流动相（缓冲液），使得柱子在你加样的时候柱子里面的凝胶或树脂没有变化，这样就不会影响你样品的分离和检测

Originally posted by cancanyeah at 2009-10-15 12:28:
The E. coli crude extract was loaded onto a DEAE 52 column equilibrated with 10 mM Tris^HCl buffer at pH 8.0 containing 0.1 mM EDTA, 1 mM dithiothreitol and 1 mM potassium cyanide.

其中的 ...