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leonumn
铁杆木虫 (著名写手)
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183BioconjugateChem.2007, 18,183?189 EncapsulatedMagneticNanoparticlesasSupportsforProteinsandRecyclable Biocatalysts AimeeR.Herdt,Byeong-SuKim,andT.AndrewTaton* UniversityofMinnesota,DepartmentofChemistry,207PleasantStreetSE,Minneapolis,Minnesota55455. ReceivedJuly14,2006;RevisedManuscriptReceivedNovember13,2006 Thispaperdescribesthebioconjugationofhistidine-taggedenzymesandotherproteinstothesurfaceofcomposite !àmagnetomicelles!–consistingofmagnet-Fe O nanoparticlesencapsulatedwithincross-linkedpolystyrene- 2 3 block-polyacrylatecopolymermicelleshells.Freecarboxylicacidgroupsonthemagnetomicellesurfacewere 2+ convertedtoCu -iminodiaceticacid(IDA)forproteincapture.TheconjugationofT4DNAligaseandenhanced greenfluorescentproteintomagnetomicellesrevealedthatproteinswerecapturedwithahighsurfacedensity andcouldbemagneticallyseparatedfromreactionmixturesandsubsequentlyreleasedfromthenanoparticle surface.Additionally,bioconjugationofT7RNApolymeraseyieldedafunctionalenzymethatmaintainedits biologicalactivityandcouldberecycledforuptothreesubsequenttranscriptionreactions.Weproposethat protein-magnetomicellebioconjugatesareeffectiveforproteinbioseparationandenzymaticrecyclingandfurther strengthentheideathatnanoparticlesurfaceshaveutilityinproteinimmobilization. |
6楼2009-04-23 08:15:22
leonumn
铁杆木虫 (著名写手)
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2楼2009-04-19 09:23:15
a9998382
木虫 (正式写手)
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3楼2009-04-19 09:29:00
leonumn
铁杆木虫 (著名写手)
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- 金币: 23565.4
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- 帖子: 1935
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- 虫号: 414915
- 注册: 2007-06-28
- 性别: GG
- 专业: 合成药物化学
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2005 Magnetomicelles:Composite Vol.5,No.10 1987-1991 NanostructuresfromMagnetic NanoparticlesandCross-Linked AmphiphilicBlockCopolymers ? ? ? ,? Byeong-SuKim, Jiao-MingQiu, Jian-PingWang, andT.AndrewTaton* DepartmentofChemistryandDepartmentofElectricalandComputerEngineering, UniVersityofMinnesota,207PleasantStreetSE,Minneapolis,Minnesota55455 ReceivedJuly19,2005;RevisedManuscriptReceivedAugust30,2005 ABSTRACT Wereportthesynthesis,characterization,andcovalentsurfacechemistryof!àmagnetomicelles!–,cross-linked,amphiphilicblock-copolym micellesthatencapsulatesuperparamagneticironoxidenanoparticles.Becausethesecompositenanostructuresassemblespontaneously fromsolutionbysimultaneousdesolvationofnanoparticleandamphiphilicpoly(styrene -block-acrylicacid )components,explicitsurface 250 13 functionalizationoftheparticlesisnotrequired,andtheencapsulationmethodwasappliedtodifferentmagneticnanoparticlesizesand compositions.TEMimagesofthemagnetomicellesillustratedthatthenumberofencapsulatedparticlescouldbedictatedrationallybysynthetic conditions.ThemagneticpropertiesoftheparticleswerecharacterizedbySQUIDmagnetometryandfollowedthegeneralLangevinmagnetic modelforsuperparamagneticmaterials.Themicellarshellsoftheseparticleswerefunctionalizedusingcovalentchemistrythatwouldnot ordinarilybepossibleonthemagneticparticlesurface.Asaresult,thisnoncovalentapproachprovidesanewroutetotechnologicalapplications ofhydrophobicmagneticnanomaterialsthatlackappropriateconjugatesurfacechemistry. |
4楼2009-04-23 08:14:16