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xuezhaoteng

金虫 (正式写手)

[求助] 审稿意见这是是要说明什么啊? 已有3人参与

What are the relevant concentrations that need to be detected in real samples?这个审稿意见是要说明什么啊,不大明白,什么意思啊?
请大虫们指点,如何去回复呢?
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godtornado

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【答案】应助回帖

感谢参与,应助指数 +1
实验样品检测的出的相关的浓度数据是什么
3楼2016-07-30 11:31:13
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yichen0405

铜虫 (小有名气)

【答案】应助回帖

感谢参与,应助指数 +1
楼主,审稿人有没有指出是在哪一段哪一句提到的这个问题呢?你是不是做了实际样的检测?
加油
2楼2016-07-30 11:16:28
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xuezhaoteng

金虫 (正式写手)

引用回帖:
2楼: Originally posted by yichen0405 at 2016-07-30 11:16:28
楼主,审稿人有没有指出是在哪一段哪一句提到的这个问题呢?你是不是做了实际样的检测?

To determine if the as-proposed method can be applied in real samples, the spiked human serum samples at three different concentrations of MTase (0.1, 1 and 10 U/mL) were analyzed using the proposed sensing assay. Here the human serum sample is without MTase (diluted 10 times with 50 mM Tris-HCl buffer) samples. The responses of the  signal for Dam MTase in diluted human serum samples were compared with those obtained from Dam MTase in buffer solution.Moreover, three independent spiked serum samples (0.1, 1 and 10 U/mL) were tested by the proposed sensing assay, and the activity of Dam MTase in the spiked serum was calculated by using the calibration curve of standard Dam MTase The recoveries of three independent spiked serum samples were found to be in the range of 95.2%-102.4%, respectively. The precision of the sensing assay was evaluated. The RSDs were in the range of 2.7%-4.5%. These results indicated the potentiality of the proposed method for detection of DNA MTase in real biological samples. 是这一段,也没看出来什么问题呢。
4楼2016-07-30 11:31:35
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xuezhaoteng

金虫 (正式写手)

引用回帖:
3楼: Originally posted by godtornado at 2016-07-30 11:31:13
实验样品检测的出的相关的浓度数据是什么

做的一个回收试验,就是加进去多少,看看用该发展的方法能检测出多少?
5楼2016-07-30 11:33:44
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