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sherry_zhang新虫 (初入文坛)
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[求助]
Small RNA-seq原始数据怎么处理？
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| 我现在有small RNA-Sequencing 的原始数据，fastq的数据格式的，我想得到miRNA 的ID名和表达信息，该怎么做啊！急求！ |
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sherry_zhang
新虫 (初入文坛)
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3楼2015-08-21 09:13:58
yingyangyang 
木虫 (正式写手)
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【答案】应助回帖
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基于小RNA-Seq数据的植物miRNA预测工具 miR-PREFeR: an accurate, fast, and easy-to-use plant miRNA prediction tool using small RNA-Seq data. Abstract Summary: Plant microRNA prediction tools that utilize small RNA sequencing data are emerging quickly. These existing tools have at least one of the following problems: 1. high false positive rate; 2. long running time; 3. work only for genomes in their databases; 4. hard to install or use. We develop miR-PREFeR (miRNA PREdiction From small RNA-Seq data), which utilizes expression patterns of miRNA and follows the criteria for plant microRNA annotation to accurately predict plant miRNAs from one or more small RNA-Seq data samples of the same species. We tested miR-PREFeR on several plant species. The results show that miR-PREFeR is sensitive, accurate, fast, and has low memory footprint. Availability: https://github.com/hangelwen/miR-PREFeR http://bioinformatics.oxfordjour ... ics.btu380.abstract |
2楼2015-08-18 15:15:24
sherry_zhang
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4楼2015-11-04 16:59:51
5楼2015-11-05 07:43:56
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