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zcc爱化学

新虫 (小有名气)

[求助] 质粒DNA在琼脂糖凝胶电泳中往负极跑 已有1人参与

如图所示,做纳米载体的基因吸附实验,载体上连接了PEI,正极在上,负极在下,空质粒DNA之前做过是正常的是向正极的,但是加了载体后的的质粒DNA集体往下(负极)跑,左边6个孔的溴酚蓝也是往负极跑的,从左往右第7个孔至第12个孔的溴酚蓝跑的方向是正常的向正极跑的,但是基因没怎么跑出来,但是有一点向下跑的趋势,有虫友知道是什么情况吗?百思不得其解,只有来求助你们啦,希望知道的友友们不吝赐教啊~~~

质粒DNA在琼脂糖凝胶电泳中往负极跑
]XU}KCD%ZF2D(N(5[R8AMXF.jpg
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genhunter

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The pore size of a 1% gel has been estimated from 100 nm to 200-500 nm.
1.         Zimm BH, Levene SD (1992). "Problems and prospects in the theory of gel electrophoresis of DNA" (PDF). Quarterly Reviews of Biophysics 25 (2): 171–204.doi:10.1017/s0033583500004662. PMID 1518924.
2.        Jump up^ Jean-Louis Viovy (2000). "Electrophoresis of DNA and other polyelectrolytes: Physical mechanisms". Reviews of Modern Physics 72: 813–872. Bibcode:2000RvMP...72..813V.doi:10.1103/RevModPhys.72.813.
离成功还差一点
5楼2015-07-16 07:40:00
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genhunter

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感谢参与,应助指数 +1
zcc爱化学: 金币+5, ★★★很有帮助 2015-07-15 17:17:53
这是由于PEI与DNA形成复合物所带的电荷性质不同(带正电)和粒径不同所致。进得了胶的分子量小些,留在孔里的是太大了,进不了胶。
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3楼2015-07-15 11:36:11
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zcc爱化学

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引用回帖:
3楼: Originally posted by genhunter at 2015-07-15 11:36:11
这是由于PEI与DNA形成复合物所带的电荷性质不同(带正电)和粒径不同所致。进得了胶的分子量小些,留在孔里的是太大了,进不了胶。

请问您知道1%的琼脂糖的孔径大概有多大吗?
4楼2015-07-15 17:18:19
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genhunter

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zcc爱化学: 金币+15 2015-07-18 09:55:00
引用回帖:
5楼: Originally posted by genhunter at 2015-07-16 07:40:00
The pore size of a 1% gel has been estimated from 100 nm to 200-500 nm.
1.         Zimm BH, Levene SD (1992). "Problems and prospects in the theory of gel electrophoresis of DNA" (PDF). Quarterly ...

2.    Jean-Louis Viovy (2000). "Electrophoresis of DNA and other polyelectrolytes: Physical mechanisms". Reviews of Modern Physics 72: 813–872. Bibcode:2000RvMP...72..813V.doi:10.1103/RevModPhys.72.813.
离成功还差一点
6楼2015-07-16 07:41:13
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