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In mitochondrion, a small portion (about 15%) of cyt c is tightly bound to the membrane while the remainder is free or loosely bound [1,2]. The unbound cyt c participates in eT and actively prevents oxidative stress,while membrane-bound cyt c ismainly involved in cell death. To initiate the apoptotic process the protein acquires peroxidase activity, an event which favors protein dissociation from the mitochondrial membrane [9,39,40] and the subsequent release of cyt c into the cytosol. Among the phospholipids constituting the mitochondrial membrane, CL is known to bind the hemoprotein [41]. Thus, the cyt c–CL interaction plays an important role in cells since it modulates the protein behavior determining whether cyt c should perform its ‘normal’ role in the respiratory chain or, conversely, is to be released into the cytosol where it participates in the apoptotic event (see [40] and references therein). Unlike the equine protein, cyt c fromyeast does not participate in cell apoptosis [16,17]. In this study we investigated the interaction of horse and yeast cyt c with CL vesicles, here considered a model for the CL-containing mitochondrial membrane, with the aim of identifying the factors distinguishing equine and yeast cyt c in their reaction with CL liposomes. The present data clearly show that only the horse cyt c–CL interaction is significantly affected by the ionic strength and ATP (both of which also exert considerable influence on the complex stability). Therefore,when the CL–cyt c binding reaction is investigated, it must be considered that c-type cyts purified from different sources may display different behavior. |
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krispanlove
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2楼2015-06-23 16:18:38
【答案】应助回帖
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爱与雨下: 金币+1 2015-07-03 20:46:18
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In mitochondrion, a small portion (about 15%) of cyt c is tightly bound to the membrane while the remainder is free or loosely bound [1,2]. The unbound cyt c participates in eT and actively prevents oxidative stress,while membrane-bound cyt c ismainly involved in cell death. To initiate the apoptotic process the protein acquires peroxidase activity, an event which favors protein dissociation from the mitochondrial membrane [9,39,40] and the subsequent release of cyt c into the cytosol. Among the phospholipids constituting the mitochondrial membrane, CL is known to bind the hemoprotein [41]. Thus, the cyt c–CL interaction plays an important role in cells since it modulates the protein behavior determining whether cyt c should perform its ‘normal’ role in the respiratory chain or, conversely, is to be released into the cytosol where it participates in the apoptotic event (see [40] and references therein). Unlike the equine protein, cyt c fromyeast does not participate in cell apoptosis [16,17]. 在线粒体中，少部分（约 15%）cyt c 紧密结合于膜上，而其他的是游离的或松散地结合的 [1,2]. 未结合的 cyt c 参与 eT，积极地防止氧化胁迫，而膜结合的 cyt c 主要参与细胞死亡。 要开始细胞凋亡过程， 蛋白获得过氧化物酶活性，这一事件促进蛋白从线粒体膜上解离 [9,39,40]，以及随后的 cyt c 释放如胞质。i在组成线粒体膜的磷脂中，已知 CL 结合血红素蛋白[41]。 因此，cyt c–CL 相互作用在细胞中有重要功能，因为它调控蛋白行为，决定cyt c 在呼吸链中行使“正常”功能，或者想反，被释放到胞质中参与凋亡事件 (参见 [40] 及其参考文献). 与马中的蛋白不同，来自酵母的 cyt c 不参与细胞凋亡 [16,17]. |
3楼2015-07-03 13:52:47