| 查看: 848 | 回复: 2 | ||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | ||
[求助]
英译汉
|
||
|
In mitochondrion, a small portion (about 15%) of cyt c is tightly bound to the membrane while the remainder is free or loosely bound [1,2]. The unbound cyt c participates in eT and actively prevents oxidative stress,while membrane-bound cyt c ismainly involved in cell death. To initiate the apoptotic process the protein acquires peroxidase activity, an event which favors protein dissociation from the mitochondrial membrane [9,39,40] and the subsequent release of cyt c into the cytosol. Among the phospholipids constituting the mitochondrial membrane, CL is known to bind the hemoprotein [41]. Thus, the cyt c–CL interaction plays an important role in cells since it modulates the protein behavior determining whether cyt c should perform its ‘normal’ role in the respiratory chain or, conversely, is to be released into the cytosol where it participates in the apoptotic event (see [40] and references therein). Unlike the equine protein, cyt c fromyeast does not participate in cell apoptosis [16,17]. In this study we investigated the interaction of horse and yeast cyt c with CL vesicles, here considered a model for the CL-containing mitochondrial membrane, with the aim of identifying the factors distinguishing equine and yeast cyt c in their reaction with CL liposomes. The present data clearly show that only the horse cyt c–CL interaction is significantly affected by the ionic strength and ATP (both of which also exert considerable influence on the complex stability). Therefore,when the CL–cyt c binding reaction is investigated, it must be considered that c-type cyts purified from different sources may display different behavior. |
回复此楼» 猜你喜欢
评审感受-评审感受-评审感受
已经有15人回复
-
本人最近太闲了，谁有问题可以提，每天会统一回复
已经有13人回复
-
这年头没有找到涵评专家，还有中面上的可能吗
已经有7人回复
-
青C资助名额大幅增加！
已经有12人回复
-
重磅！青年科学基金项目（C类）资助增幅预计超过50%
已经有6人回复
-
有带发论文的吗
已经有3人回复
-
导师各种操作恶心咋办
已经有13人回复
-
护理论文 晋升
已经有5人回复
-
求助大佬sci投稿哪个好中
已经有4人回复
-
2026博士申请求助
已经有10人回复
krispanlove
金虫 (小有名气)
- 应助: 26 (小学生)
- 金币: 719.4
- 红花: 21
- 帖子: 254
- 在线: 29.1小时
- 虫号: 3898944
- 注册: 2015-05-30
- 性别: MM
- 专业: 环境工程
2楼2015-06-23 16:18:38
【答案】应助回帖
★ ★ ★ ★ ★ ★
爱与雨下: 金币+1 2015-07-03 20:46:18
不辜负: 金币+5, 翻译EPI+1, ★★★很有帮助, 谢谢你噢 2015-07-08 01:19:58
爱与雨下: 金币+1 2015-07-03 20:46:18
不辜负: 金币+5, 翻译EPI+1, ★★★很有帮助, 谢谢你噢 2015-07-08 01:19:58
|
In mitochondrion, a small portion (about 15%) of cyt c is tightly bound to the membrane while the remainder is free or loosely bound [1,2]. The unbound cyt c participates in eT and actively prevents oxidative stress,while membrane-bound cyt c ismainly involved in cell death. To initiate the apoptotic process the protein acquires peroxidase activity, an event which favors protein dissociation from the mitochondrial membrane [9,39,40] and the subsequent release of cyt c into the cytosol. Among the phospholipids constituting the mitochondrial membrane, CL is known to bind the hemoprotein [41]. Thus, the cyt c–CL interaction plays an important role in cells since it modulates the protein behavior determining whether cyt c should perform its ‘normal’ role in the respiratory chain or, conversely, is to be released into the cytosol where it participates in the apoptotic event (see [40] and references therein). Unlike the equine protein, cyt c fromyeast does not participate in cell apoptosis [16,17]. 在线粒体中，少部分（约 15%）cyt c 紧密结合于膜上，而其他的是游离的或松散地结合的 [1,2]. 未结合的 cyt c 参与 eT，积极地防止氧化胁迫，而膜结合的 cyt c 主要参与细胞死亡。 要开始细胞凋亡过程， 蛋白获得过氧化物酶活性，这一事件促进蛋白从线粒体膜上解离 [9,39,40]，以及随后的 cyt c 释放如胞质。i在组成线粒体膜的磷脂中，已知 CL 结合血红素蛋白[41]。 因此，cyt c–CL 相互作用在细胞中有重要功能，因为它调控蛋白行为，决定cyt c 在呼吸链中行使“正常”功能，或者想反，被释放到胞质中参与凋亡事件 (参见 [40] 及其参考文献). 与马中的蛋白不同，来自酵母的 cyt c 不参与细胞凋亡 [16,17]. |
3楼2015-07-03 13:52:47