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论文题目：Toxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae)
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Toxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae).
作者:Liang, Yaping; Li, Xiuwei; Gu, Zumin; Qin, Peiwen; Ji, Mingshan
Molecules (Basel, Switzerland)
卷:20 期:2 页:3238-54
DOI:10.3390/molecules20023238
出版年:2015 Feb 16
摘要
The larvicidal activity of the crude petroleum ether, ethyl acetate, acetone, chloroform and ethanol extracts of Amorpha fruticosa seeds was individually assayed for toxicity against the early fourth-instar larva of the mosquito, Culex pipiens pallens after 24 h exposure. Of the tested extracts, the ethanol one exhibited the highest larvicidal activity (LC50 = 22.69 mg/L). Amorphigenin (8'-hydroxyrotenone), a rotenoid compound which exhibits a strong larvicidal activity with LC50 and LC90 values of 4.29 and 11.27 mg/L, respectively, was isolated from the ethanol extract by column chromatograpy. Its structure was elucidated by 1H-NMR, UV and IR spectral data. Furthermore, investigation of amorphigenin's effects on mitochondrial complex I activity and protein synthesis in C. pipiens pallens larvae reveals that amorphigenin decreases mitochondrial complex I activities to 65.73% at 10.45 mumol/L, compared to the control, when NADH were used as the substrate. Meanwhile, amorphigenin at 10.45 mumol/L also caused a 1.98-fold decrease in protein content, compared to the control larvae treated with acetone only.
作者信息
地址

epartment of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China. lypnancy628@163.com.
文献信息
文献类型:Journal Article
语种:English
PubMed ID:25690287
NLM 唯一 ID:100964009
创建日期: 18 Feb 2015
电子出版:16 Feb 2015
国家/地区: Switzerland
ISSN:1420-3049
期刊信息
Impact Factor (影响因子): Journal Citation Reports®
其他信息
分主题子库:Index Medicus
状态:In-Data-Review

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