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ÂÛÎÄÌâÄ¿£ºToxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae)
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Toxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae).
×÷Õß:Liang, Yaping; Li, Xiuwei; Gu, Zumin; Qin, Peiwen; Ji, Mingshan
Molecules (Basel, Switzerland)
¾í:20 ÆÚ:2 Ò³:3238-54
DOI:10.3390/molecules20023238
³ö°æÄê:2015 Feb 16
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The larvicidal activity of the crude petroleum ether, ethyl acetate, acetone, chloroform and ethanol extracts of Amorpha fruticosa seeds was individually assayed for toxicity against the early fourth-instar larva of the mosquito, Culex pipiens pallens after 24 h exposure. Of the tested extracts, the ethanol one exhibited the highest larvicidal activity (LC50 = 22.69 mg/L). Amorphigenin (8'-hydroxyrotenone), a rotenoid compound which exhibits a strong larvicidal activity with LC50 and LC90 values of 4.29 and 11.27 mg/L, respectively, was isolated from the ethanol extract by column chromatograpy. Its structure was elucidated by 1H-NMR, UV and IR spectral data. Furthermore, investigation of amorphigenin's effects on mitochondrial complex I activity and protein synthesis in C. pipiens pallens larvae reveals that amorphigenin decreases mitochondrial complex I activities to 65.73% at 10.45 mumol/L, compared to the control, when NADH were used as the substrate. Meanwhile, amorphigenin at 10.45 mumol/L also caused a 1.98-fold decrease in protein content, compared to the control larvae treated with acetone only.
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µØÖ·epartment of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China. lypnancy628@163.com.
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ÎÄÏ×ÀàÐÍ:Journal Article
ÓïÖÖ:English
PubMed ID:25690287
NLM Ψһ ID:100964009
´´½¨ÈÕÆÚ: 18 Feb 2015         
µç×Ó³ö°æ:16 Feb 2015
¹ú¼Ò/µØÇø: Switzerland
ISSN:1420-3049
ÆÚ¿¯ÐÅÏ¢
Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports®
ÆäËûÐÅÏ¢
·ÖÖ÷Ìâ×Ó¿â:Index Medicus
״̬:In-Data-Review
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