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ÂÛÎÄÌâÄ¿£ºToxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae) Çë°ïæ²éѯ¸ÃÂÛÎÄÊÇ·ñ±»SCI¼ìË÷ лл |
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sunshan4379: ½ð±Ò+2, ¸ÐлӦÖú£¡SCIÊÕ¼ÊÇÖ¸web of science ºËÐĺϼ¯ÖÐµÄ 2015-03-12 11:07:22
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Toxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae). ×÷Õß:Liang, Yaping; Li, Xiuwei; Gu, Zumin; Qin, Peiwen; Ji, Mingshan Molecules (Basel, Switzerland) ¾í:20 ÆÚ:2 Ò³:3238-54 DOI:10.3390/molecules20023238 ³ö°æÄê:2015 Feb 16 ÕªÒª The larvicidal activity of the crude petroleum ether, ethyl acetate, acetone, chloroform and ethanol extracts of Amorpha fruticosa seeds was individually assayed for toxicity against the early fourth-instar larva of the mosquito, Culex pipiens pallens after 24 h exposure. Of the tested extracts, the ethanol one exhibited the highest larvicidal activity (LC50 = 22.69 mg/L). Amorphigenin (8'-hydroxyrotenone), a rotenoid compound which exhibits a strong larvicidal activity with LC50 and LC90 values of 4.29 and 11.27 mg/L, respectively, was isolated from the ethanol extract by column chromatograpy. Its structure was elucidated by 1H-NMR, UV and IR spectral data. Furthermore, investigation of amorphigenin's effects on mitochondrial complex I activity and protein synthesis in C. pipiens pallens larvae reveals that amorphigenin decreases mitochondrial complex I activities to 65.73% at 10.45 mumol/L, compared to the control, when NADH were used as the substrate. Meanwhile, amorphigenin at 10.45 mumol/L also caused a 1.98-fold decrease in protein content, compared to the control larvae treated with acetone only. ×÷ÕßÐÅÏ¢ µØÖ· epartment of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China. lypnancy628@163.com.ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Journal Article ÓïÖÖ:English PubMed ID:25690287 NLM Ψһ ID:100964009 ´´½¨ÈÕÆÚ: 18 Feb 2015 µç×Ó³ö°æ:16 Feb 2015 ¹ú¼Ò/µØÇø: Switzerland ISSN:1420-3049 ÆÚ¿¯ÐÅÏ¢ Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ÆäËûÐÅÏ¢ ·ÖÖ÷Ìâ×Ó¿â:Index Medicus ״̬:In-Data-Review |

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epartment of Pesticide Science, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
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