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qiuzhen8809

新虫 (小有名气)

[交流] 请问293F细胞是293 Freestyle cell吗? 293F细胞如何养呢?

请问293F细胞是293 Freestyle cell吗? 293F细胞如何养呢?
好像可以贴壁培养也可以悬浮培养,分别是怎么培养的?
非常感谢!!
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清月城堡

金虫 (正式写手)


小木虫: 金币+0.5, 给个红包,谢谢回帖
用特定培养基,Gibco有专用培养基
8楼2022-04-25 22:56:48
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蓝天_1988

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★ ★ ★
小木虫: 金币+0.5, 给个红包,谢谢回帖
qiuzhen8809: 金币+1, F是fast growing的意思吗? 与invitrogen的freestyle 293 2015-03-10 13:47:16
qiuzhen8809: 金币+1, F是fast growing的意思吗? 与invitrogen的freestyle 293没有关系吗? 2015-03-10 13:47:59
293-F and 293-H Cells are clonal isolates, derived from transformed embryonal human kidney cells (1,2). The 293-F strain is a fast-growing variant of the 293 cell line. The 293-H strain is a variant of the 293 cell line that demonstrates better adherence in monolayer culture and ease of use for plaque assays and other anchorage dependent applications.
The 293T cells are derived from 293 cells but stably express the SV40 large T antigen which can bind to SV40 enhancers of expression vectors to increase protein production.
293T are also neomycin resistant due to the presence of a neomycin resistance cassete together with the SV40 Large T .
In addition to the comments above, the presence of T-antigen in 293T cells could help in episomal maintenance of SV40 origin containing vectors. In case of viral production and for other transfections 293T is now preferred since they are normally better transfectable.
3楼2015-03-10 13:28:24
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qiuzhen8809

新虫 (小有名气)

引用回帖:
3楼: Originally posted by 蓝天_1988 at 2015-03-10 13:28:24
293-F and 293-H Cells are clonal isolates, derived from transformed embryonal human kidney cells (1,2). The 293-F strain is a fast-growing variant of the 293 cell line. The 293-H strain is a variant  ...

F是fast growing的意思吗? 与invitrogen的freestyle 293没有关系吗?
4楼2015-03-10 13:50:56
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肖颖2009

铁虫 (正式写手)


小木虫: 金币+0.5, 给个红包,谢谢回帖
293F 是悬浮细胞,使用的培养基为Gibco的Freestyle 293F培养基。改细胞在加血清的条件下也可以贴壁培养。
5楼2015-05-27 16:35:23
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