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永峰1230

铜虫 (小有名气)

[求助] 序列提交 reading frame shifts 已有1人参与

序列提交后收到邮件:
We have noticed that some or all of the sequences contain internal stop
codons and/or reading frame shifts (insertions/deletions based on BLAST
similarity search results and/or an alignment).

This generally indicates problems such as:

  - errors in the nucleotide sequence
  - sequences have not been trimmed of low quality
  - problems with feature annotation which may be due to:
        translation in the wrong reading frame or on the wrong strand
        translation does not have the correct exon spans
        translation includes noncoding sequence, such as introns or UTR's
          
If the sequences and/or annotation are incorrect, please resubmit
with the corrected files. If the information is correct, please explain
the presence of the internal stop codons and/or frame shifts.

If you are unable to determine the correct sequence and/or annotation
for your sequence(s), they can still be processed and the following
comment will be added to your records:

COMMENT  GenBank staff is unable to verify sequence and/or annotation
         provided by the submitter.
         
In addition, the record(s) will indicate that the data are unverified and
your sequence(s) will not be included in NCBI BLAST databases.

Please either:

        [1] resubmit with correct sequence(s) and/or annotation
        or
        [2] confirm that your sequence(s) should be processed as unverified.
试过好几种方法,,都是这种邮件,,,该怎么弄呢???
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车彦辰

新虫 (初入文坛)

引用回帖:
8楼: Originally posted by 永峰1230 at 2015-01-24 08:43:27
还没有了, 回复邮件还是那样子,  这几天忘记弄了。。...

求助啊,我的也是这样啊。你的解决了么???
9楼2015-04-03 16:30:53
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