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永峰1230铜虫 (小有名气)
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[求助]
序列提交 reading frame shifts 已有1人参与
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序列提交后收到邮件： We have noticed that some or all of the sequences contain internal stop codons and/or reading frame shifts (insertions/deletions based on BLAST similarity search results and/or an alignment). This generally indicates problems such as: - errors in the nucleotide sequence - sequences have not been trimmed of low quality - problems with feature annotation which may be due to: translation in the wrong reading frame or on the wrong strand translation does not have the correct exon spans translation includes noncoding sequence, such as introns or UTR's If the sequences and/or annotation are incorrect, please resubmit with the corrected files. If the information is correct, please explain the presence of the internal stop codons and/or frame shifts. If you are unable to determine the correct sequence and/or annotation for your sequence(s), they can still be processed and the following comment will be added to your records: COMMENT GenBank staff is unable to verify sequence and/or annotation provided by the submitter. In addition, the record(s) will indicate that the data are unverified and your sequence(s) will not be included in NCBI BLAST databases. Please either: [1] resubmit with correct sequence(s) and/or annotation or [2] confirm that your sequence(s) should be processed as unverified. 试过好几种方法，，都是这种邮件，，，该怎么弄呢？？？ |
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wizardfan
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永峰12302楼2014-12-20 10:20:26
永峰1230
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3楼2014-12-30 10:09:07
wizardfan
至尊木虫 (著名写手)
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【答案】应助回帖
★ ★ ★ ★ ★ ★
永峰1230: 金币+6, ★★★很有帮助 2014-12-31 11:02:54
永峰1230: 金币+6, ★★★很有帮助 2014-12-31 11:02:54
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unverified = 没进一步证实, it can be verified by you or someone else. But as it is a new sequence, you are the only person to verify it. If I see an unverified sequence in NCBI, I won't use it as the quality of the sequence is unknown. |
4楼2014-12-31 10:43:16
永峰1230
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5楼2014-12-31 11:02:46
wizardfan
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【答案】应助回帖
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永峰1230: 金币+8, ★★★很有帮助, 谢谢你，，thank you 2015-01-03 11:56:18
永峰1230: 金币+8, ★★★很有帮助, 谢谢你，，thank you 2015-01-03 11:56:18
| according to the content of NCBI email, it should be fine to submit your sequence while confirming they are unverified. But there is no point for doing so. It is up to you. The only thing you will get is a NCBI accession for your sequence, that is it, probably no one will use it. |
6楼2014-12-31 20:52:16
7楼2015-01-23 23:26:49
永峰1230
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8楼2015-01-24 08:43:27
9楼2015-04-03 16:30:53
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您好！我也遇到了Internal stop codons have been detected in the conceptual translation of the following coding regions这个问题，请问您是怎么解决的，毕业在即，文章等着序列释放才接受。望慷慨相助。yxx20060429@163.com |
10楼2018-04-11 21:39:03
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