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01062032

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[求助] 帮我!跪谢!!

哪位大神可以帮我翻译一下,跪谢。。还有那个plug 在这里是什么意思。。
Here, we present an implementation of KSEC-MS, in which short plugs of SM and P are injected sequentially into a SEC column without the need to premix P and SM outside the column. We call this implementation plug−plug KSEC-MS (ppKSEC-MS) in analogy with plug−plug kinetic capillary electrophoresis.
  这篇文章的摘要是:
     
Studying the kinetics of reversible protein−small molecule binding is a major challenge. The available approaches require that either the small molecule or the protein be modified by labeling or immobilization on a surface. Not only can such modifications be difficult to do but also they can drastically affect the kinetic parameters of the interaction.
To solve this problem, we present kinetic size-exclusion chromatography with mass spectrometry detection (KSECMS), a solution-based label-free approach. KSEC-MS utilizes
the ability of size-exclusion chromatography (SEC) to separate any small molecule from any protein−small molecule complex without immobilization and the ability of mass spectrometry (MS) to detect a small molecule without a label. The rate constants of complex formation and dissociation are deconvoluted from the temporal pattern of small molecule elution measured with MS at the exit from the SEC column. This work describes the concept of KSEC-MS and proves it in principle by measuring the rate constants of interaction between carbonic anhydrase and acetazolamide.
这是篇关于什么的文章大体解释一下。。。。谢谢
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