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lixingyu: ½ð±Ò+10, ¡ï¡ï¡ïºÜÓаïÖú 2014-11-08 10:44:34
sunshan4379: LS-EPI+1, ¸ÐлӦÖú£¡ 2014-11-12 10:26:00
lixingyu: ½ð±Ò+10, ¡ï¡ï¡ïºÜÓаïÖú 2014-11-08 10:44:34
sunshan4379: LS-EPI+1, ¸ÐлӦÖú£¡ 2014-11-12 10:26:00
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ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1 ×÷Õß:Li, XY (Li, Xing-Yu)[ 1 ] ; Mao, ZC (Mao, Zi-Chao)[ 1 ] ; Wang, YH (Wang, Yue-Hu)[ 2 ] ; Wu, YX (Wu, Yi-Xing)[ 1 ] ; He, YQ (He, Yue-Qiu)[ 1 ] ; Long, CL (Long, Chun-Lin)[ 2,3 ] JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY ¾í: 22 ÆÚ: 2 Ò³: 83-93 DOI: 10.1159/000338530 ³ö°æÄê: 2012 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª Bacillus subtilis XF-1 (CGMCC No. 2357), a patent strain with good effects for controlling the clubroot of crucifer and many pathogenic fungi, was predicted to produce cyclic lipopeptide (CLP) antibiotics based on its genomic analysis. In this study, the CLPs were purified and determined with the following protocol: the supernatant of XF-1 cultivating mixture was firstly precipitated, then the precipitants were extracted with methanol and further separated by Sephadex LH-20 chromatography to test its antifungal activities. Fungi-inhibiting fractions were further characterized with LC/ESI-MS and LC/ESI-MS/MS. The results show that four molecular ion peaks [M+H](+) (m/z 1,464, 1,478, 1,492 and 1,506) from fungi suppression fraction were identified as fengycin A with fatty acid of C-16-C-19, fengycin B (C-14-C-17), fengycin C (C-15-C-18), fengycin D (C-15-C-18) and fengycin S (C-15-C-18). Fengycin C (C-15 and C-18), fengycin D (C-15, C-16 and C-18) and fengycin S (C-15, C-16 and C-18) were reported for the first time. The diversity of the fengycins that exist in this strain will help the elucidation of their biocontrol mechanisms. Copyright (C) 2012 S. Karger AG, Basel ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Bacillus subtilis XF-1; Fengycins; Antifungal fraction; LC/ESI-MS/MS KeyWords Plus ESORPTION IONIZATION-TIME; PEPTIDE ENZYME-INHIBITORS; FLIGHT-MASS-SPECTROMETRY; STRUCTURAL-CHARACTERIZATION; AMYLOLIQUEFACIENS FZB42; RAPID IDENTIFICATION; WHOLE-CELL; FENGYCIN; SURFACTIN; GLOEOSPORIOIDES×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: He, YQ (ͨѶ×÷Õß) Yunnan Agr Univ, Key Lab Agr Biodivers & Pest Control, Kunming 650201, Peoples R China. µØÖ·: [ 1 ] Yunnan Agr Univ, Key Lab Agr Biodivers & Pest Control, Kunming 650201, Peoples R China [ 2 ] Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China [ 3 ] Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China µç×ÓÓʼþµØÖ·:ynfh2007@163.com; long@mail.kib.ac.cn »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ Ministry of Agriculture of China Special Fund for Agro-Scientific Research in the Public Interest 2010029030 Natural Science Foundation of Yunnan Province 2008CC024 National Natural Science Foundation of China 20972166 31070288 31161140345 Ministry of Education of China B08044 MUC 985-6 MUC 98506-01000101 ²é¿´»ù½ð×ÊÖúÐÅÏ¢ ³ö°æÉÌ KARGER, ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND Àà±ð / ·ÖÀà Ñо¿·½Ïò:Biotechnology & Applied Microbiology; Microbiology Web of Science Àà±ð:Biotechnology & Applied Microbiology; Microbiology ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000306883100002 PubMed ID: 22614917 ISSN: 1464-1801 ÆÚ¿¯ÐÅÏ¢ Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ÆäËûÐÅÏ¢ IDS ºÅ: 980HB Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 36 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 2 |
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