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lixingyu

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单位要填表，需要CSI文章的检索号，我上不了web of science,请大家帮我查查，非常感谢！
Xing-Yu Li, Zi-Chao Mao, Yue-Hu Wang, Yi-Xing Wu, Yue-Qiu He, Chun-Lin Long. ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1. Journal of Molecular Microbiology and Biotechnology. 2012, 22: 83-93. DOI: 10.1159/000338530

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baiyuefei

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lixingyu: 金币+10, ★★★很有帮助 2014-11-08 10:44:34
sunshan4379: LS-EPI+1, 感谢应助！ 2014-11-12 10:26:00

ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1

作者:Li, XY (Li, Xing-Yu)[ 1 ] ; Mao, ZC (Mao, Zi-Chao)[ 1 ] ; Wang, YH (Wang, Yue-Hu)[ 2 ] ; Wu, YX (Wu, Yi-Xing)[ 1 ] ; He, YQ (He, Yue-Qiu)[ 1 ] ; Long, CL (Long, Chun-Lin)[ 2,3 ]

JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

卷: 22

期: 2

页: 83-93

DOI: 10.1159/000338530

出版年: 2012

查看期刊信息

摘要

Bacillus subtilis XF-1 (CGMCC No. 2357), a patent strain with good effects for controlling the clubroot of crucifer and many pathogenic fungi, was predicted to produce cyclic lipopeptide (CLP) antibiotics based on its genomic analysis. In this study, the CLPs were purified and determined with the following protocol: the supernatant of XF-1 cultivating mixture was firstly precipitated, then the precipitants were extracted with methanol and further separated by Sephadex LH-20 chromatography to test its antifungal activities. Fungi-inhibiting fractions were further characterized with LC/ESI-MS and LC/ESI-MS/MS. The results show that four molecular ion peaks [M+H](+) (m/z 1,464, 1,478, 1,492 and 1,506) from fungi suppression fraction were identified as fengycin A with fatty acid of C-16-C-19, fengycin B (C-14-C-17), fengycin C (C-15-C-18), fengycin D (C-15-C-18) and fengycin S (C-15-C-18). Fengycin C (C-15 and C-18), fengycin D (C-15, C-16 and C-18) and fengycin S (C-15, C-16 and C-18) were reported for the first time. The diversity of the fengycins that exist in this strain will help the elucidation of their biocontrol mechanisms. Copyright (C) 2012 S. Karger AG, Basel

关键词

作者关键词:Bacillus subtilis XF-1; Fengycins; Antifungal fraction; LC/ESI-MS/MS

KeyWords Plus

ESORPTION IONIZATION-TIME; PEPTIDE ENZYME-INHIBITORS; FLIGHT-MASS-SPECTROMETRY; STRUCTURAL-CHARACTERIZATION; AMYLOLIQUEFACIENS FZB42; RAPID IDENTIFICATION; WHOLE-CELL; FENGYCIN; SURFACTIN; GLOEOSPORIOIDES

作者信息

通讯作者地址: He, YQ (通讯作者)

Yunnan Agr Univ, Key Lab Agr Biodivers & Pest Control, Kunming 650201, Peoples R China.

地址:

[ 1 ] Yunnan Agr Univ, Key Lab Agr Biodivers & Pest Control, Kunming 650201, Peoples R China

[ 2 ] Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China

[ 3 ] Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China

电子邮件地址:ynfh2007@163.com; long@mail.kib.ac.cn

基金资助致谢

基金资助机构

授权号

Ministry of Agriculture of China Special Fund for Agro-Scientific Research in the Public Interest

2010029030

Natural Science Foundation of Yunnan Province

2008CC024

National Natural Science Foundation of China

20972166

31070288

31161140345

Ministry of Education of China

B08044

MUC 985-6

MUC 98506-01000101

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出版商

KARGER, ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND

类别 / 分类

研究方向:Biotechnology & Applied Microbiology; Microbiology

Web of Science 类别:Biotechnology & Applied Microbiology; Microbiology

文献信息

文献类型:Article

语种:English

入藏号: WOS:000306883100002

PubMed ID: 22614917

ISSN: 1464-1801

期刊信息

Impact Factor (影响因子): Journal Citation Reports®

其他信息

IDS 号: 980HB

Web of Science 核心合集中的 "引用的参考文献": 36

Web of Science 核心合集中的 "被引频次": 2

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