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【答案】应助回帖
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lixingyu: 金币+10, ★★★很有帮助 2014-11-08 10:44:34
sunshan4379: LS-EPI+1, 感谢应助！ 2014-11-12 10:26:00
ESI LC-MS and MS/MS Characterization of Antifungal Cyclic Lipopeptides Produced by Bacillus subtilis XF-1
作者:Li, XY (Li, Xing-Yu)[ 1 ] ; Mao, ZC (Mao, Zi-Chao)[ 1 ] ; Wang, YH (Wang, Yue-Hu)[ 2 ] ; Wu, YX (Wu, Yi-Xing)[ 1 ] ; He, YQ (He, Yue-Qiu)[ 1 ] ; Long, CL (Long, Chun-Lin)[ 2,3 ]
JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY
卷: 22
期: 2
页: 83-93
DOI: 10.1159/000338530
出版年: 2012
查看期刊信息
摘要
Bacillus subtilis XF-1 (CGMCC No. 2357), a patent strain with good effects for controlling the clubroot of crucifer and many pathogenic fungi, was predicted to produce cyclic lipopeptide (CLP) antibiotics based on its genomic analysis. In this study, the CLPs were purified and determined with the following protocol: the supernatant of XF-1 cultivating mixture was firstly precipitated, then the precipitants were extracted with methanol and further separated by Sephadex LH-20 chromatography to test its antifungal activities. Fungi-inhibiting fractions were further characterized with LC/ESI-MS and LC/ESI-MS/MS. The results show that four molecular ion peaks [M+H](+) (m/z 1,464, 1,478, 1,492 and 1,506) from fungi suppression fraction were identified as fengycin A with fatty acid of C-16-C-19, fengycin B (C-14-C-17), fengycin C (C-15-C-18), fengycin D (C-15-C-18) and fengycin S (C-15-C-18). Fengycin C (C-15 and C-18), fengycin D (C-15, C-16 and C-18) and fengycin S (C-15, C-16 and C-18) were reported for the first time. The diversity of the fengycins that exist in this strain will help the elucidation of their biocontrol mechanisms. Copyright (C) 2012 S. Karger AG, Basel
关键词
作者关键词:Bacillus subtilis XF-1; Fengycins; Antifungal fraction; LC/ESI-MS/MS
KeyWords Plus ESORPTION IONIZATION-TIME; PEPTIDE ENZYME-INHIBITORS; FLIGHT-MASS-SPECTROMETRY; STRUCTURAL-CHARACTERIZATION; AMYLOLIQUEFACIENS FZB42; RAPID IDENTIFICATION; WHOLE-CELL; FENGYCIN; SURFACTIN; GLOEOSPORIOIDES
作者信息
通讯作者地址: He, YQ (通讯作者)
Yunnan Agr Univ, Key Lab Agr Biodivers & Pest Control, Kunming 650201, Peoples R China.
地址:
[ 1 ] Yunnan Agr Univ, Key Lab Agr Biodivers & Pest Control, Kunming 650201, Peoples R China
[ 2 ] Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
[ 3 ] Minzu Univ China, Coll Life & Environm Sci, Beijing, Peoples R China
电子邮件地址:ynfh2007@163.com; long@mail.kib.ac.cn
基金资助致谢
基金资助机构
授权号
Ministry of Agriculture of China Special Fund for Agro-Scientific Research in the Public Interest
2010029030
Natural Science Foundation of Yunnan Province
2008CC024
National Natural Science Foundation of China
20972166
31070288
31161140345
Ministry of Education of China
B08044
MUC 985-6
MUC 98506-01000101
查看基金资助信息
出版商
KARGER, ALLSCHWILERSTRASSE 10, CH-4009 BASEL, SWITZERLAND
类别 / 分类
研究方向:Biotechnology & Applied Microbiology; Microbiology
Web of Science 类别:Biotechnology & Applied Microbiology; Microbiology
文献信息
文献类型:Article
语种:English
入藏号: WOS:000306883100002
PubMed ID: 22614917
ISSN: 1464-1801
期刊信息
Impact Factor (影响因子): Journal Citation Reports®
其他信息
IDS 号: 980HB
Web of Science 核心合集中的 "引用的参考文献": 36
Web of Science 核心合集中的 "被引频次": 2 |
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