| 查看: 1828 | 回复: 4 | ||
[求助]
有没有人知道detergent-resistant membranes 已有2人参与
|
| 细胞膜上有抗去垢剂区域吗？detergent-resistant membranes，有没有大神懂得给解释一下，抗去垢剂膜和病毒内吞有何联系？因为一篇文章中说病毒的一些非结构蛋白会和抗去垢剂膜相联系，感谢。。。 |
回复此楼» 猜你喜欢
评审感受-评审感受-评审感受
已经有15人回复
-
本人最近太闲了，谁有问题可以提，每天会统一回复
已经有13人回复
-
这年头没有找到涵评专家，还有中面上的可能吗
已经有7人回复
-
青C资助名额大幅增加！
已经有12人回复
-
重磅！青年科学基金项目（C类）资助增幅预计超过50%
已经有6人回复
-
有带发论文的吗
已经有3人回复
-
导师各种操作恶心咋办
已经有13人回复
-
护理论文 晋升
已经有5人回复
-
求助大佬sci投稿哪个好中
已经有4人回复
-
2026博士申请求助
已经有10人回复
2楼2014-06-12 19:16:49
【答案】应助回帖
|
History • 80’s Order (package) and disorder phases in diff. parts of the PM. • 90’s Analysis of membrane fractions by the use of mild detergents. The concept of Detergent Resistant Membranes (DRM) born. In this technique non-solubilized fraction of the PM extracts by mild detergent (triton X-100 at 4ºC –artifact conditions-) + sucrose gradient • 2000’s New microscopy techniques: FRET, STED, FRAP… To understand the dynamic of the assembly of sterol, sphingolipds and proteins in living cells (real conditions). Membrane domain definition: Lateral compartment (PM plane) with composition, structure and biological function diff. from the surrounding membrane. Regardless size, stability and mechanism of formation. Why DRMs/DIMs (detergent insoluble microdomains) are not lipid rafts??? • 4ºC is not a real condition for the behavior of the PM. • Quantitative bias due the ratio concentration of detergent:prot. /detergent:lipids Definition by consensus 2006: “Membrane rafts are small (10-200nm), heterogeneous highly dynamic, sterol and sphingolipid-enriched domains that compartmentalized cellular process” What is new here: No: detergent resistant, lipid term, or PM (E.g. membrane of mitochondria), small (need of superesolution techniques). |
3楼2014-06-12 19:20:22
4楼2019-06-04 12:17:08
5楼2019-06-04 12:21:45