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shimaohu123

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[求助] 双指数拟合荧光寿命怎么分析

Calculated using 2 exponentials

Prompt data : Prompt
Decay data : Decay

The initial paramters are:

Shift Value = 0 ch
            0 sec
Shift Limit = 40 ch
            8.77915E-09 sec

T1 Estimate = 11.57449 ch
            2.540355E-09 sec
T2 Estimate = 46.29797 ch
            1.016142E-08 sec

A Free
B1 Free
B2 Free

Prompt and decay LO = 404 ch
                     8.866941E-08 sec
Prompt and decay HI = 647 ch
                     1.420027E-07 sec

Background on prompt = 3
Time calibration = 2.194787E-10 sec/ch
---------------------------------------------
The fitted parameters are:

Hi reduced to: 607 ch

SHIFT = 0.8839028 ch
      1.939979E-10 sec
      S.Dev = 3.790324E-12 sec

T1 = 12.29505 ch
   2.698503E-09 sec
   S.Dev = 1.407488E-10 sec
T2 = 18.58244 ch
   4.07845E-09 sec
   S.Dev = 1.984843E-11 sec

A  = 7.525081
   S.Dev = 0.44061

B1 = -0.2825815
   [-72.85 Rel.Ampl]
   S.Dev = 2.110813E-03
B2 = 0.4436326
   [172.85 Rel.Ampl]
   S.Dev = 1.557658E-03

CHISQ = 1.186022
[ 198 degrees of freedom ]

Chi-squared Probability = 3.752265%
Durbin-Watson Parameter = 1.950561
Negative residuals    = 48.52941%
Residuals < 1 s.dev    = 64.21568%
Residuals < 2 s.dev    = 93.13725%
Residuals < 3 s.dev    = 99.5098%
Residuals < 4 s.dev    = 99.5098%

双指数是两个寿命，怎么分析呢，里面的参数怎么看

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