| 查看: 1552 | 回复: 5 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
hanamichi木虫 (小有名气)
|
[交流]
Acc. Chem. Res.综述：含DNA功能化纳米材料在细胞水平上生物传感/成像/靶向治疗的应用 已有1人参与
|
||
|
Functional DNA-Containing Nanomaterials: Cellular Applications in Biosensing, Imaging, and Targeted Therapy Hao Liang †, Xiao-Bing Zhang *†, Yifan Lv †, Liang Gong †, Ruowen Wang †, Xiaoyan Zhu †, Ronghua Yang *†, and Weihong Tan *†‡ † Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Collaborative Innovation Center of Molecular Engineering for Theranostics, Hunan University, Changsha, Hunan 410082, China ‡ Center for Research at Bio/nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, UF Genetics Institute, and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611-7200, United States  Conspectus DNA performs a vital function as a carrier of genetic code, but in the field of nanotechnology, DNA molecules can catalyze chemical reactions in the cell, that is, DNAzymes, or bind with target-specific ligands, that is, aptamers. These functional DNAs with different modifications have been developed for sensing, imaging, and therapeutic systems. Thus, functional DNAs hold great promise for future applications in nanotechnology and bioanalysis. However, these functional DNAs face challenges, especially in the field of biomedicine. For example, functional DNAs typically require the use of cationic transfection reagents to realize cellular uptake. Such reagents enter the cells, increasing the difficulty of performing bioassays in vivo and potentially damaging the cell’s nucleus. To address this obstacle, nanomaterials, such as metallic, carbon, silica, or magnetic materials, have been utilized as DNA carriers or assistants. In this Account, we describe selected examples of functional DNA-containing nanomaterials and their applications from our recent research and those of others. As models, we have chosen to highlight DNA/nanomaterial complexes consisting of gold nanoparticles, graphene oxides, and aptamer–micelles, and we illustrate the potential of such complexes in biosensing, imaging, and medical diagnostics. Under proper conditions, multiple ligand–receptor interactions, decreased steric hindrance, and increased surface roughness can be achieved from a high density of DNA that is bound to the surface of nanomaterials, resulting in a higher affinity for complementary DNA and other targets. In addition, this high density of DNA causes a high local salt concentration and negative charge density, which can prevent DNA degradation. For example, DNAzymes assembled on gold nanoparticles can effectively catalyze chemical reactions even in living cells. And it has been confirmed that DNA–nanomaterial complexes can enter cells more easily than free single-stranded DNA. Nanomaterials can be designed and synthesized in needed sizes and shapes, and they possess unique chemical and physical properties, which make them useful as DNA carriers or assistants, excellent signal reporters, transducers, and amplifiers. When nanomaterials are combined with functional DNAs to create novel assay platforms, highly sensitive biosensing and high-resolution imaging result. For example, gold nanoparticles and graphene oxides can quench fluorescence efficiently to achieve low background and effectively increase the signal-to-background ratio. Meanwhile, gold nanoparticles themselves can be colorimetric reporters because of their different optical absorptions between monodispersion and aggregation. DNA self-assembled nanomaterials contain several properties of both DNA and nanomaterials. Compared with DNA–nanomaterial complexes, DNA self-assembled nanomaterials more closely resemble living beings, and therefore they have lower cytotoxicity at high concentrations. Functional DNA self-assemblies also have high density of DNA for multivalent reaction and three-dimensional nanostructures for cell uptake. Now and in the future, we envision the use of DNA bases in making designer molecules for many challenging applications confronting chemists. With the further development of artificial DNA bases using smart organic synthesis, DNA macromolecules based on elegant molecular assembly approaches are expected to achieve great diversity, additional versatility, and advanced functions. |
回复此楼» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : ar500078f.pdf
2014-04-30 22:52:25, 7.99 M
» 本帖已获得的红花（最新10朵）
hongyanjiang» 猜你喜欢
温州大学王娟课题组2026年招生
已经有10人回复
-
温州大学2026年招生（化学、材料）
已经有10人回复
-
无机化学论文润色/翻译怎么收费?
已经有138人回复
-
招生负责_院士团队_学生高自由度成长_擅长学生培养
已经有5人回复
-
温州大学招收2026年入学博士研究生（化学、材料、环境）
已经有10人回复
-
非晶合金
已经有2人回复
-
院士团队-每届学生都优秀-高自由度-团队氛围好
已经有0人回复
-
求调剂
已经有2人回复
-
化工学院（学院正式调剂群+联系方式）
已经有0人回复
-
化工学院（学院官方群）-院士团队招生-学生自由度高-培养模式成熟高效
已经有0人回复
» 本主题相关商家推荐: (我也要在这里推广)
» 本主题相关价值贴推荐，对您同样有帮助:
- 张希院士Acc. Chem. Res.最新综述：基于多功能生物界面的主客体作用的界面超分子化学
已经有64人回复
- Acc. Chem. Res.最新综述：无定型的金属——有机骨架
已经有63人回复
- Acc. Chem. Res. 最新综述：水溶液中的刺激响应性超分子聚合物——田禾院士出品
已经有28人回复
- Acc. Chem. Res. 热点综述：基于石墨烯的有机合成
已经有35人回复
- 江雷院士Acc. Chem. Res.最新综述:具有优越力学性能的Bioinspired Layered Materials
已经有46人回复
- Acc. Chem. Res.最新综述：Switchable Host−Guest Systems on Surfaces
已经有17人回复
- Acc. Chem. Res.最新综述Functional Polymeric Microparticles Engineered
已经有10人回复
- YASHIMA的Acc. Chem. Res.经典综述:Single- and Double-Stranded Helical Polymers
已经有15人回复
- Acc. Chem. Res综述-“Homeopathic”纳米Pd用于碳碳偶联反应+纳米金综述2篇
已经有29人回复
- 厦门大学Acc. Chem. Res.最新综述：具有高能晶面的金属氧化物微纳晶体及其应用
已经有322人回复
- Acc. Chem. Res.2013 唐本忠院士最新发表有关AIE（聚集诱导荧光）的综述
已经有216人回复
- Acc. Chem. Res.上关于核壳结构的最新综述
已经有782人回复
- Acc Chem Res 最新综述 : 石墨烯发展的安全顾虑 - 碳纳米管的教训
已经有249人回复
- Acc Chem Res 综述 : 石墨烯纳米复合材料在太阳能转换，储存，传感方面的前景
已经有131人回复
- Acc Chem Res 研究进展：形状控制Pd纳米催化及其催化应用
已经有69人回复
- Acc. Chem. Res.最新综述：具有介孔-大孔多级纳米结构的碳材料---设计、表征及应用
已经有465人回复
- Acc. Chem. Res.最新综述：碳基催化剂---氧化石墨烯及其衍生物
已经有230人回复
- Acc. Chem. Res.最新综述：核-壳结构纳米催化剂
已经有718人回复
- Acc. Chem. Res.最新综述：基于大面积高质量石墨烯薄膜的杂化功能系统
已经有151人回复
- Acc. Chem. Res.最新综述：用于太阳能转化、存储、传感的石墨烯基复合物的设计与展望
已经有214人回复
- Acc. Chem. Res.最新综述：纳米石墨烯和石墨烯边缘---电子结构以及纳米制造
已经有100人回复
- Acc. Chem. Res. 新综述--量子点的细胞毒性及减小毒性的方法
已经有119人回复
- John B. Goodenough在Acc. Chem. Res.上的最新综述-现代可充电电池的策略演化
已经有440人回复
- 张希院士在Acc. Chem. Res.上刚出的好综述：超两亲自组装
已经有604人回复
- Acc. Chem. Res.最新综述：高指数晶面贵金属纳米晶
已经有101人回复
0.8
| 顶一下，感谢分享！ |
4楼2014-05-01 17:07:14
0.8
| 顶一下，感谢分享！ |
2楼2014-05-01 14:27:31
nowitzki_ci
专家顾问 (著名写手)
-
专家经验: +139 - MN-EPI: 17
- 应助: 67 (初中生)
- 金币: 21504.8
- 散金: 220
- 红花: 60
- 帖子: 2325
- 在线: 1505.9小时
- 虫号: 1035941
- 注册: 2010-06-04
- 性别: GG
- 专业: 高分子合成化学
- 管辖: 微米和纳米
3楼2014-05-01 15:17:16
0.8
| 顶一下，感谢分享！ |
5楼2014-05-01 21:24:36
