16楼: Originally posted by 夏菡小屋 at 2014-04-21 09:10:31
我查过细菌的好想大概是这样的大小范围，植物的也是这样吗？ 用普通的胶跑的有影响吗？网上有报道说 植物的在4kb左右。...

12楼: Originally posted by 夏菡小屋 at 2014-04-21 09:03:36
不对啊  我是根据文献的操作来的啊。参考文献是：A simple and rapid method for RNA isolation from plant tissues with high phenolic compounds and polysaccharides.
Extraction buffer: 0.25 M NaCl, 0.05 M ...

14楼: Originally posted by 夏菡小屋 at 2014-04-21 09:07:07
我用过天根的植物总RNA提取试剂盒来提取，没提取出来。...

18楼: Originally posted by Allen206 at 2014-04-21 09:42:31
如果不从大小上分辨，能看出来哪个是DNA哪个是RNA 么？...

20楼: Originally posted by fanwq258 at 2014-04-21 10:43:55
这种SDS法主要是用于提取核酸的，主要是DNA，当然，RNA也能够提取出来，但是质量不太好，你还得去除基因组DNA。建议你还是用trizol法提RNA吧。...

22楼: Originally posted by gyesang at 2014-04-21 12:04:14
我看到的A simple and rapid method for RNA isolation from plant tissues with high phenolic compounds and polysaccharides
这个方法，跟你提供的完全不一样，你自己可以去对比下...

25楼: Originally posted by grape_vine at 2014-04-21 16:32:07
他这个生姜的材料，我估计是数据多糖多酚含量比较高的，用trizol应该搞不定！...

26楼: Originally posted by 夏菡小屋 at 2014-04-21 16:38:51
那你能把你看到的发我一下嘛？ 我说的这个方法 完全是从文献中copy 出来的。不知道哪出问题了啊？...

28楼: Originally posted by gyesang at 2014-04-22 00:01:44
A simple and rapid method for RNA isolation from plant tissues with high phenolic compounds and polysaccharides
放google一搜就出来了啊，要学会去查啊...