不对啊 我是根据文献的操作来的啊。参考文献是：A simple and rapid method for RNA isolation from plant tissues with high phenolic compounds and polysaccharides.
Extraction buffer: 0.25 M NaCl, 0.05 M Tris-HCl (pH 7.5), 20 mM EDTA, 1% (w/v) sodium dodecyl sulphate (SDS) and 4% (w/v) PVP (M.W. 360,000)
RNA extraction
1 Add 7.5 ml of extraction buffer and 7.5 ml of CI to a 30 ml-round bottom Nalgene tube.
2 Grind 1 g frozen mangosteen sample to fine powder with a mortar and pestle in liquid nitrogen.
3 Transfer 1 g of ground sample to a tube containing the extraction buffer and CI and vortex vigorously.
4 Centrifuge at 12,857 g for 2 min at room temperature.
5 Transfer the supernatant to a new 30 ml-round bottom Nalgene tube and purify with an equal volume of PCI. Centrifuge at 12,857 g for 2 min at room temperature. Repeat this step until there
is a clean interface observed.
6 Transfer the supernatant to a new 30 ml-round bottom Nalgene tube and add one tenth volume of 3 M sodium acetate pH 5.2 and 2.5 volume of cold absolute ethanol, mix well, and incubate at 4 °C for 30 min.
7 Recover the nucleic acids by centrifugation at 12,857 g for 20 min at 4 °C.
8 Wash the pellet with 70% (v/v) ethanol, air-dry, and add 200 μl DEPC-treated water to dissolve the pellet.
9 Transfer the supernatant to a 1.5 ml tube and add 10 M LiCl to a final concentration of 2 M and keep on ice for 1 h. Centrifuge at 18,514 g for 20 min at 4 °C.
10 Wash the pellet with 70% (v/v) ethanol, air-dry, and add 20 μl DEPC-treated water to
dissolve the pellet. Centrifuge at 18,514 g for 10 min at 4 °C.
11 Add 0.1 volume of 3 M sodium acetate pH 5.2 and 2.5 volume of cold absolute ethanol, mix well, and store at -80 °C.
Analysis of RNA quality
12. The total RNA was quantified with a spectrophotometer at 230, 260, 280 nm. The integrity of total RNA was verified by analyzing approximately 1 μg RNA sample on 1% (w/v) formaldehyde denaturing agarose gel
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