| 查看: 772 | 回复: 1 | ||||
qiuqinglei5020
|
[求助]
阿哌沙班液相分析条件？
|
| 有做阿哌沙班的吗？液相条件帮忙说一下 |
回复此楼» 收录本帖的淘帖专辑推荐
 新药研发注册申报 |
» 猜你喜欢
药学考研调剂
已经有0人回复
-
华南理工大学-医学院-药学专硕少量调剂名额
已经有28人回复
-
药物学论文润色/翻译怎么收费?
已经有224人回复
-
求助《生物利用度和生物等效性研究指导原则》（试 行），2002
已经有0人回复
-
文献求助
已经有0人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- 岛津液相色谱一台，如何对仪器和分析条件进行设置，使峰面积增大？？
已经有11人回复
- 液相色谱测定苯酚的浓度的条件及柱子类型
已经有12人回复
- 【求助】关于有机盐类农药的液相检测时液相色谱柱选择及其色谱条件
已经有8人回复
- 液相色谱的安装条件要求，特别是环境要求
已经有5人回复
- 液相色谱分离条件的优化
已经有9人回复
- 苯酐（邻苯二甲酸酐）液相分析的条件！！！
已经有14人回复
- 胡萝卜素液相条件：安捷伦1260反相C18色谱柱
已经有14人回复
- 求助：五氯酚钠的高效液相色谱的条件？
已经有6人回复
- 样品检测 不知道液相条件怎么选择？？谢谢！！！
已经有5人回复
- 【求助】液相色谱色谱条件选择问题，谢谢！
已经有3人回复
- 【求助】求助：在ESI离子源条件下，液相流动相的配制应注意哪些问题?
已经有8人回复
- 【求助】液相分离条件筛选出来后，进行质谱分析，质谱响应程度的影响因素有哪些呢？
已经有5人回复
- 【求助】液质联用分析中对液相色谱条件的流动相有什么具体的要求？
已经有4人回复
yanghuping
木虫 (正式写手)
- 应助: 100 (初中生)
- 金币: 5103.5
- 散金: 2331
- 红花: 8
- 沙发: 1
- 帖子: 939
- 在线: 1099.3小时
- 虫号: 965040
- 注册: 2010-03-08
- 性别: GG
- 专业: 有机合成
【答案】应助回帖
感谢参与，应助指数 +1
|
有一篇文献上面的分析条件：General Experimental: All reagents used were of ACS grade or higher. Flash chromatography was performed using either a Biotage Flash Chromatography System or an AnaLogix BSR Solvent Pump System with silica gel cartridges. HPLC analyses for the syntheses were performed on an Agilent 1100 HPLC system including solvent degasser, pump, automated injector and variable UV detector connected to either an IN/US BetaRam Flow Detector with a 0.25ml detector cell or a Berthold Scintillation Detector with a 0.5 ml detector cell. HPLC gradient 1 = Phenomenex Luna C18, 5 mm, 4.6150mm column. Mobile phase A = 0.05% TFA in water, mobile phase B = 0.05% TFA in acetonitrile, 0min 95% A, 6min 50% A, 11 min 95% B, 13 min 95% B, 15 min 95% A, UV detection at 254 nm. HPLC gradient 2 = Vydac protein and peptide C18, 4.6250mm column. Mobile phase A= 0.05% TFA in water, mobile phase B = 0.05% TFA in acetonitrile, 0min 90% A, 16 min 90% B, 23 min 95% B, 25 min 90% A, UV detection at 254 nm. HPLC Gradient 3 = Phenomenex, Luna, C18 (2), 3 mm, 1504.6mm column with Brownlee, RP-18 C18, 3 mm, 153.2mm guard column, column temperature = 301C, autosampler temperature = 41C, mobile phase A = 0.4% formic acid in water adjusted to pH 3.2 with NH4OH, mobile phase B = acetonitrile, mobile phase C = MeOH, 0 min 100% A, 25 min 20% A and 80% B, 27min 50% B and 50% C, 32 min 50% B and 50% C, 34 min 100% A, flowrate = 1.0ml/min at 323 nm. HPLC Gradient 4 =Ace 3, C18, 3 mm, 1504.6mm column with Ace 3, C18, 3 mm, 103.0mm guard column, column temperature = 351C, autosampler temperature = ambient, mobile phase A = 0.4% formic acid in water, adjusted to pH 3.2 with NH4OH, mobile phase B = acetonitrile, 0min 100% A, 3min 100% A, 5min 90% A, 20 min 75% A, 50 min 75% A, 60 min 50% A, 65 min 100% B, 70 min 100% B, 72 min 100% A, flowrate = 0.7ml/min, UV detection at 240 nm. Preparative HPLC condition 1: Phenomenex Luna 5 mm, 25021.2mm column. Mobile phase A = 0.1% TFA in water, mobile phase B = 0.1% TFA in acetonitrile, 0 min 70% A, 18 min 95% B, 22 min 95% B, 25 min 70% A, flowrate = 15 ml/min. UV detection at 254 nm. Preparative HPLC condition 2: Zorbax SB-C18 5 mm, 25021.2mm column. Mobile phase A = 0.05% TFA in water, mobile phase B = 0.05% TFA in acetonitrile, 0 min 90% A, 16 min 90% B, 23 min 95% B, 25 min 90% A, Flowrate = 9.9ml/min, UV detection at 254 nm. LC/MS analysis was performed on a Finnigan LXQ Mass Spectrometer System. NMR spectra were recorded on a Bruker AVANCE II 300MHz Spectrometer with an UltrashieldTM Magnet or a Varian Unity/Inova 300MHz Spectrometer. Specific activities were performed gravimetrically. Liquid scintillation counting was performed on either a PerkinElmer Tri-Carb Model 2900Tr or a Wallac Model 1409 Liquid Scintillation Counter. |
» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : 1890_ftp.pdf
2013-10-17 15:46:21, 998.45 K
2楼2013-10-17 15:43:13
