[求助] 氨氧化细菌的片段长度

2楼: Originally posted by 承卿此诺 at 2013-07-19 22:33:05
length of fragment: 491bp.
PCR: 95C 1min; 40*(95C 30s, 55C 30s, 72C 1min, plate read 85C); 72C 10min.
primer: amoA 1F+amoA 2R
E=90%~105%
You can find details in ISI or Google Scholar with key wor ...

3楼: Originally posted by zx1990 at 2013-07-20 08:22:28
Thank you so much!And if it easy to be amplified in this condition?...

4楼: Originally posted by 承卿此诺 at 2013-07-20 11:44:41
Yes, the amplication efficiecy is 90%~105%....

2楼: Originally posted by 承卿此诺 at 2013-07-19 22:33:05
length of fragment: 491bp.
PCR: 95C 1min; 40*(95C 30s, 55C 30s, 72C 1min, plate read 85C); 72C 10min.
primer: amoA 1F+amoA 2R
E=90%~105%
You can find details in ISI or Google Scholar with key wor ...

6楼: Originally posted by zx1990 at 2013-08-05 08:02:50
请问你说的491bp是包括前后引物的长度的吗？因为我从克隆结果得到的片段长度是452bp，加上前后引物的长度才是491...

7楼: Originally posted by 承卿此诺 at 2013-08-10 16:12:21
加上引物才是491bp。...

9楼: Originally posted by 小风车 at 2013-11-27 20:07:45
各位大侠好，我最近做这个amoA的扩增，一直都没有特异性条带，你说的这个条件我也试过，还是没有结果。我采用的是50ul的体系，用的PCR MIX，引物跟你的一样。采用的是从好氧颗粒污泥中提取的DNA为模板，求解答，感激 ...