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zx1990

金虫 (小有名气)

[求助] 氨氧化细菌的片段长度

求教诸位大神,安氧化细菌的片段长度是多少?一般PCR的条件和体系分别是怎样的?还有所用的引物,速求,最近在做实验,不知道氨氧化细菌好不好P?
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木虫 (小有名气)

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3楼: Originally posted by zx1990 at 2013-07-20 08:22:28
Thank you so much!And if it easy to be amplified in this condition?...

Yes, the amplication efficiecy is 90%~105%.
4楼2013-07-20 11:44:41
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木虫 (小有名气)

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zx1990(gyesang代发): 金币+2, 鼓励回帖应助! 2013-07-20 12:03:46
length of fragment: 491bp.
PCR: 95C 1min; 40*(95C 30s, 55C 30s, 72C 1min, plate read 85C); 72C 10min.
primer: amoA 1F+amoA 2R
E=90%~105%
You can find details in ISI or Google Scholar with key words "ammonia-oxidizing bacteria".

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2楼2013-07-19 22:33:05
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zx1990

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2楼: Originally posted by 承卿此诺 at 2013-07-19 22:33:05
length of fragment: 491bp.
PCR: 95C 1min; 40*(95C 30s, 55C 30s, 72C 1min, plate read 85C); 72C 10min.
primer: amoA 1F+amoA 2R
E=90%~105%
You can find details in ISI or Google Scholar with key wor ...

Thank you so much!And if it easy to be amplified in this condition?
3楼2013-07-20 08:22:28
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zx1990

金虫 (小有名气)

引用回帖:
4楼: Originally posted by 承卿此诺 at 2013-07-20 11:44:41
Yes, the amplication efficiecy is 90%~105%....

OK, thanks!
5楼2013-07-21 07:56:55
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