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wds_111

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[求助] 葡聚糖凝胶层析问题 已有1人参与

我买了葡聚糖凝胶G50,先纯化一个蛋白(分子量10000左右),我用沸水浴溶胀了2小时,然后装柱上样,结果怎么也洗脱不下来,这是怎么回事?
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zizzozizzo

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【答案】应助回帖

我认为有两个可能性:1 沸水溶胀,有可能个破坏凝胶的结构,一般选择常温下24小时,条件比较温和对凝胶比较好。2 你装柱后应当检查,看是否有断层。
2楼2014-04-29 10:02:19
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