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E.coli. 1.DnaAÏȰ󵽽ÐdnaA boxesµÄÆðʼλµãÉÏ£¬È»ºóÓëRNA¾ÛºÏøºÍHUµ°°×Ï໥×÷Óá£Ç°ÕßÊÇÓÃÀ´ºÏ³ÉÆðʼDNA¸´ÖƵÄÒýÎºóÕßÊÇΪÁËÈÃdnaA box×ó¶ËµÄµÄ²¿·ÖË«Á´ÈÚ½âΪµ¥Á´¡£ 2.È»ºóDnaBµ°°×°óµ½¿ª·ÅµÄ¸´ºÏÎïÉÏ£¨ÈÚ½âΪµ¥Á´µÄÇøÓò£©ÉÏ£¬¸øÒýÎï°óµ½DNAÄ£°åÁ´Ìá¹²ÁË·½±ã¡£DnaBÒ²ÓнâÐýø»îÐÔ£¬¿ÉÒÔʹ˫Á´±äΪµ¥Á´¡£Ðγɵĵ¥Á´ÎªÁ˱ÜÃâÓÖ³ÉΪ˫Á´£¬ÍùÍù»á°óÉϵ¥Á´½áºÏµ°°×£¨SSB£©. 3.DNA ºËÐÄøͨ¹ýÓÉÁ½¸öbetaÑÇ»ù×é³ÉµÄ»¬¶¯»·½áºÏµ½DNAÄ£°åÁ´ÉϽøÐи´ÖÆ¡£ÕæºËÉúÎïµÄ»¬¶¯»·ÓÉÈý¸öbetaÑÇ»ù×é³É¡£ |

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| The pol III holoenzyme is double-headed, with two core polymerases attached through two ¦Ó-subunits to a g complex. One core is responsible for (presumably) continuous synthesis of the leading strand, the other performs discontinuous synthesis of the lagging strand. The g complex serves as a clamp loader to load the b clamp onto a primed DNA template. Once loaded, the b clamp loses affi nity for the g complex and associates with the core polymerase to help with processive synthesis of an Okazaki fragment. Once the fragment is completed, the b clamp loses affi nity for the core polymerase and associates with the g complex, which acts as a clamp unloader, removing the clamp from the DNA. Then it can recycle to the next primer and repeat the process. |

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