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[求助]
对一个基因的生物信息学分析
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| 最近刚刚完成了一个基因的克隆，用以做基因敲除，现在想写一篇有关该基因的克隆及生物信息学分析，请问我应该分析那些方面，用到那些软件呢 |
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【答案】应助回帖
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感谢参与，应助指数 +1
wizardfan: 金币+2, 谢谢分享经验 2013-05-04 07:56:35
感谢参与，应助指数 +1
wizardfan: 金币+2, 谢谢分享经验 2013-05-04 07:56:35
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Sequence analysis, multiple sequences alignment and phylogenetic analysis The searches for nucleotide and protein sequence similarities were conducted with BLAST algorithm at the National Center for Biotechnology Information (NCBI, http://www.ncbi.nlm.gov/blast). The deduced amino acid sequence of EsCypA was analyzed with the Expert Protein Analysis System (Expasy, http://www.expasy.org/). SignalP 3.0 program was utilized to predict the presence and location of signal peptide (http://www.cbs.dtu.dk/services/SignalP/). The protein motif features were predicted by Simple Modular Architecture Research Tool (SMART) version 7.0 (http://smart.embl-heidelberg.de/). Multiple sequence alignment of EsCypA and other reported Cyps was performed with ClustalW multiple alignment program (http://www.ebi.ac.uk/Tools/clustalw2/) and multiple alignment show program (http://www.bioinformatics.org/sms/). An unrooted phylogenic tree was constructed based on the deduced amino acid sequences of EsCypA and other reported Cyps by the neighbor-joining (NJ) algorithm using the MEGA 5.05 software (http://www.megasoftware.net). To derive the confidence value for the phylogeny analysis, bootstrap trials were replicated 1000 times. from my paper-The molecular characterization of A cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein |
2楼2013-05-02 18:08:08
3楼2013-05-03 16:47:32
