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冰蓝色的泪珠

新虫 (初入文坛)

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虫号: 2278904
注册: 2013-02-10
专业: 生物化学

[求助] 求问枯草杆菌蛋白酶与地衣芽孢杆菌区别？

需要一种酶解血浆中样品与巯基相连的部分参考一篇文献用sigma公司的枯草杆菌蛋白酶  目前在sigma网站上找到这个p5380 来自于地衣芽孢杆菌求问一样吗？
P5380
MSDS
Protease 来源于地衣芽孢杆菌
Type VIII, lyophilized powder, 7-15 units/mg solid (Sigma)
o
o CAS号 9014-01-1
o    EINECS 编号 232-752-2
o    Enzyme Commission (EC) Number 3.4.21.62  ( BRENDA  | IUBMB  )
o    MDL number MFCD00132092

PROTEASE, BACTERIAL, TYPE VIII
Sigma Prod. No. P 5380
CAS NUMBER: 9014-01-1
ENZYME COMMISSION NO.: 3.4.21.62
SYNONYMS: Subtilisin A, Subtilisin Carlsberg, Subtilopeptidase A
STRUCTURE:
It is a single polypeptide chain of MW 27,287 (complete amino acid sequence available on request).2
PHYSICAL DESCRIPTION:
Isoelectric point: pI = 9.4
E1%(280nm) = 8.6
Sedimentation coefficient: S20,w = 2.773
STABILITY / STORAGE AS SUPPLIED:
This protease is offered as a white powder, essentially free of buffer salts. It is quite stable stored frozen.
Supplier studies indicate that the solid will lose less than 10% of its activity per year if stored at 4°C. Longterm
storage in excess of 20°C is to be avoided.
SOLUBILITY / SOLUTION STABILITY:
The product is readily soluble in water at all concentrations which occur in normal usage, to give a
colorless solution.2 Sigma tests it in 10 mM sodium acetate buffer, containing 5 mM calcium acetate, pH
7.5, for assay purposes, and obtains a clear solution.1
Subtilisin A is reportedly active in some organic solvents, dry octane, for example.4
This enzyme is stable 1-2 days at 4°C as a 100-200 mg/mL solution in 0.1 M borate, pH 8.0, containing
0.1 M calcium chloride.3

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