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hanbin7686

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[求助] 请问大家爱有谁知道停乳链球菌的培养基如何配置么？谢谢各位的帮助了

现在正在做链球菌有关的实验，请问大家知道链球菌如何培养么？期待回复。
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daiybe

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daiybe

【答案】应助回帖

感谢参与，应助指数 +1
146??????????
THE COMPOSITION OF MEDIA
???????????

1. Acetobacter Medium (???????????)
Glucose (??????) 100g Yeasst extract (?????) 10g
CaCO3 20g Agar (???) 15g
Distilled water (?????) 1000ml Adjust (??) pH to 6.8
???÷?Χ??????????????????

2. Nutrient Agar (?????????)
Pepton (??????) 5g Beef extract (????) 30g
NaCl 5g Agar (???) 15g
Distilled water (?????) Adjust (??) pH to 7.0-7.2
[Note]??When cultivation of Bacillus??5mg of to MnSO4.H2O may be added .
It is favorable to promote spore formation .
???÷?Χ?????????????????????????????????????????????????????????????????????????????????????????????С????????????????????????????????????????????????????????????????????????????????????????????????????±???????????????????????????????????????????????????????????????????????????????????????????????????????μ??????????????????????????????????????????????????????????????????????????????????????????????????????????????

3. Azotobacter Medium (???????????)
KH2PO4 0.2g K2HPO4 0.8g
MgSO4.7H2O 0.2g CaSO4.2H2O???? 0.1??
????????a2MoO4.2H2O Trace(???) Yeast axtract(?????) 0.5g
Mannitol(?????) 20g FeCl3 Tract(???)
Distilled water (?????) 1000ml Agar (???) 15g
Adjust (??) pH to 7.2
???÷?Χ??????????????????

4. Corn Meal Medium (???????????)
Maize flour (?????) 5g Peptone (??????) 0.1g
Glucose (??????) 1g Tap water (?????) 1000ml
[Note]:Boil the mixture in autoclave at 121?? for 1 hr. distribute the medium into 18??18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121?? for 1 hr . again (15??????1С?,?????18??18???????,???????6?????15????????15С???)

5. Lactic-bacteria Medium I (??????????? I )
Yeast extract (?????) 7.5g Peptone (??????) 7.5g
Glucose (??????) 10g KH2PO4 2g
Tomato juice (???????) 100ml Tween (????) 80 0.5ml
Distilled water (?????) 900ml pH 7.0
???÷?Χ???????????????????????????????????

6. Lactic-bacteria Midium ?? (??????????? ??)
Lacto-casein peptone (?????????) 10g Beef extract (??????) 10g
Yeast extract (?????) 5g Glucose (??????) 5g
Tween (????) 80 1g K2HPO4 2g
Na-acetate (??????) 5g Diamine citrate (?????????) 2g
MgSO4.7H2O 0.2g MnSO4.H2O 0.05g
Distilled water (?????) 1000m pH 6.5-6.8
???÷?Χ????????????????????

7. Peotone Glucose Yeast extract Medium PGY ????????????????????????????
Peptone????????? 10g Yeast extract ??????? 5g
Glucose ????????? 1g Distilled water ????????? 1L
8. Glycerol Agar ???????????
Peptone ????????? 5g Beef extract ??????? 3g
Glycerol ??????? 20g Top water ????????? 1000ml
Agar ??????? 15g pH 7.0-7.2

9. Rhizobium medium ????????????????AS 9
Yeast eztract ??????? 1g Soil eztract ????????????? 200ml
Mannitol ????????? 10g Agar ??????? 15g
Distilled water ????????? 800ml pH 7.2
[Note]??Soil extract??Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121?? for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121?? , then mixed with othringredients and distributed. (?????????????????????50??????200??????15??????1С???????????????????200??????)
???÷?Χ?????????????????????????????????????????????????????????????
?????????????????????????????????????????????????????????

10. Mannitol Agar (????????)
Yeast extract (?????) 5g Peptone (??????) 3g
Mannitol (?????) 25g Agar (???) 15g
Distilled water (?????) 1000ml

11. Glucose Asparagine ?????????????????????
Glucose ????????? 10g Asparagine ?????????? 0.5g
K2HPO4 0.5g Water ????? 1000ml
pH 7.2-7.4
???÷?Χ??????С?????筺????????С???????筺?С???????



12. Gause??s Synthetic Agar ????????????????
KNO3 1g Soluble starch???????????? 20g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4 0.01g
Agar ??????? 20g water ????? 1000ml
pH 7.2-7.4
???÷?Χ??????С?????筺????????С???????筺?С??????????????ù?????????ù??????????ù????????????ù?????????ù??????????ù???????????ù??????????ù??????ù????ù?????????ù??????????ù?????????ù?????????ù?????????ù???????????ù?????????????ù??????????ù?????????ù?????????ù??????????ù??????????ù??????????ù?????????????ù???????????ù?????????ù?????????ù??

13. Wort Agar ???????????
Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then distribute the medium into tubes. Autoclave at 110 for 30 minutes. (??????????????δ?????????????12??????????15????????????15?????30?????)
???÷?Χ?????????????????????????????????????????????????????????????????????????????????????????????????ü?????????????????????ù???????ù????ù?????????????????????????????????????????????????????????????????????????????????????????С????????????????????????????????????????????????????????????????????????????????????????????????????????·???????????????????????????????????????????????????????ù???????ù???????ù??????ù???????ù?????????ù??????ù??

14. Potato Dextrose Agar PDA (?????????????????)
Potato extract (???????) 1000ml Dextrose(glucose) (??????) 20g
Agar (???) 20g
[Note]: Potato extract: Slice potatoes thin. Add 1000ml of water, immediately to prevent Oxidtion of juice and boil until soft. (approximately 30min.). Filter through cotton-cloth. Autoclave at 115?? for 20 minutes.??????????????200????г?С?飬???1000???????30???????????????飬???????????1000??????????????20??????15????????????15?????30???????
???÷?Χ??????????????????????????????????ù??????????ù?????????ù
??????????ù?????????ù??????????ù????ɡ????ù?????·????ù??????ù??
????ù????????ù?????ù????????????????ù????????????????????魰?
?????????????????????????????????????????????ù?????????????
?????????????????????????????????????????????????????????????
?????????ù??С????ù??????????????????ù???????????ù???????ù????
??ù?????????ù??????ù???????ù??????ù?????ù?????????ù?????
?ù??????ù????????ù????????????????????????????ù?????????
??ù??????ù?????θ????????????????ù??????ù???????ù????????ù???????ù??????ù?????ù???????ù????[ù?????????ù???????ù???????ù??????ù??????????????????????



15. Czapek??s Agar???????????
Sucrose ??????? 30g NaNO3 3g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.7H2O 0.01g K2HPO4 1g
Agar (???) 13g Distilled water (?????) 1000ml
pH 7.2
???÷?Χ????ù????ù?????????ù???????ù???????ù???????ù??????????????ù???????ù???????ù??????ù?????????ù???????ù??????ù??????ù????????ù??????ù??????ù??????ù????????ù????????ù??????ù??????????ù??????ù???????ù????????ù????????ù????????ù????????ù??????ù??????ù???????ù???????ù???????ù??????ù????????ù????????ù????????ù????????ù???????ù??С????ù???????ù??

16. Concentrated Sucrose Czapek??s (?????????)
Sucrose (????) 200g NaNO3 3g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.4H2O 0.01g K2HPO4 1g
Agar (???) 13g Distilled water (?????) 1000ml
???÷?Χ??л????ù

17. Synthetic Potato Medium (???????????????)
20%Potato extract (???????) 1L Glucose (??????) 20g
KH2PO4 3g MgSO4.7H2O 1.5g
Vitanib B1 (????) Trace (???) Agar (???) 15g
pH 6
???÷?Χ??????ù????????ù????????ù??????????ù????????ù?????ù????????????????ù?????????????????? ????????魰?????????????????????????????????????????????????????????????????ù?????????????????????????????????????ù?????????????????????????????????????????????????????ù??????ù?????????ù??????ù???????ù??????ù?????ù?????????ù??????ù??????ù????????ù????????????????????????????ù???????????ù???????????ù??????ù??????????????ù????ù???????????ù???????ù????????ù??????ù???????ù??????ù?????ù??????ù???????ù?????ù???????ù?????????ù????ù?????????[ù???????ù??????ù???⿵???ù???ù??????ù??????ù??????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????漧????????С?ɡ?????????????ɡ???????????????????????????????????????????????????????ɡ???????ɡ????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????ζ??????????????????????????????β????????????????????????????????????????????????????????????????????????????????????????????????????????С??????????


18. Corn Meal Medium (???????????)
Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into 100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at 121?? for 30minutes . (20%?????????15?????30?????)

19. Filter Paper Medium ?????????????
??NH4??2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.7g NaCl 0.5g
Distilled water ????????? 1000ml A strip of filter paper ????????? 6??1cm ???????

20. Soybean Cake Meal Agar (??????????)
10% infusion of soybean cake meal (10%?????????) 1000ml
Glucose (??????) 10g NaCl 2.5g

21. Sawdust?? Wheat-bran Medium ???м?????????????
Sawdust of broad leaf tree 75g Wheat bran 25g
Preparation??Mix these two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth .
?????????????????м 75?? ?????????25??
????????????????????????????????????????????????????????????С??????????????20?????1С???

22. Pine Block or Pine Sowdust Medium ????????????м????????
1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (??10??2??1????????????????1-2%????????С?????????????????????????????????20?????1С???)
2. Pine Sawdust Medium
Pine Sawdust (???м) 75% Rice bran (???) 23-24%
Socrose (????) 1-2% Water (appropriate)? ????????
Autoclave at 20 lbs for 1 hr.(20?????1С???

23. Rice Straw. Dung Medium (???????????????)
Dried horse dung (or cow dung) ???????????? 65%
Died straw ???????? 35% Urea ??????? 0.5%
Calcium superhosphate ??????????0.7% Gypsum powder??????? 1%
Ammonium sulphate ??????泥? 0.3%
[Note]: Add appropriate water, pile them up and cause them fermentation. ??????????ж???????

24. Straw Rice Bran Medium ??????????????????
Dried straw ???????? 75-80% Rice bran ??????? 20-25%
[Note]??Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [?]?????ù??????г???3?????С?Σ??????????12С???????????????????????????????????????????????????????)

25. Rice Medium (?????????)
Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121?? for 30 minutes (or fill 2g of rice into a 15??150mm tube). Add 6 ml of water. Autoclave at 121?? for 30 minutes. (?????10?????50?????????У??20-30??????15?????30???????????????2??????15??150????????У????6????????15?????30?????)

26. Filter Paper Medium (???????????)
(NH4)2SO4 1g KH2PO4 1g
???? MgSO4.7H2O 0.5g Yeast Extract (?????) 0.1g
Distilled Water (?????) 1000ml
Strip of filter paper 1??7 cm (????? 1??7 ????)
[Note]??Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121?? for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (??????????????????????????????1??????15?????30????????????????????1????2???????????м?????á?)

27. Yeast Extract-Medium Extract Agar I (?????????????)
Yeast extract (?????) 10g Mall extract (?????) 10g
Glucose (??????) 4g Distilled water (?????) 1000ml
Agar (???) 20g pH 7.0
???÷?Χ????ù????ù??

28. Yeast Extract Peptone ??????????????????
Yeast extract ??????? 1g Multi-peptone????????? 2g
Beef extract ?????? 1g Glucose ????????? 10g
Agar ??????? 20g Distilled water ????????? 1L
pH 7.0

29. Glucose Yeast Extract Starch Agar ???????????????????????
Glucose ????????? 10g Yeast extract ??????? 10g
Starch ??????? 10g NaCl 5g
CaCO3 3g Agar ??????? 20g
Distilled water ????????? 1L pH 7.0

30. Wheat Bran Medium ?????????????
Wheat bran ??????? 36g ??NH4??2HPO4 10g
K2HPO4 0.2g MgSO4??7H2O 0.1g
Agar ??????? 20g Distilled water ????????? 1L
pH 7.0



31. Yeast Sporulation Medium (???????????????????????)
(1) Kleyn Medium
KH2PO4 0.012% K2HPO4 0.02%
Sodium acetate (??????) 0.5% Glucose (??????) 0.062%
NaCl 0.062% Peptone (??????) 0.25%
Biotin (??????) 2??g% Agar (???) 2%
Trace element solution (???????) 1ml/100ml
[Note]: Trace element solution ???????????????? MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%

(2) Gorodkowa Medium
Glucose (??????) 0.1% Peptone (??????) 1%
NaCl 0.5% Agar (???) 2%

(3) McClary Medium
Glucose (??????) 0.1% KCl 0.18%
Yeast juice (????) 0.25% Sodium actate (??????) 0.82%
Agar (???) 1.5%

32. BPY Medium (BPY ??????)
Beef extract (????) 5g Prptone (??????) 10g
NaCl 5g Yeast extract (?????) 5g
Glucose (??????) 5g Agar (???) 1-2%
Distilled (?????) 1000ml pH 7.0
???÷?Χ?????????????????????????????

33. LB Medium (LB ??????)
Yeast extact (?????) 5g Peptone (??????) 10g
NaCl 10g Agar (???) 1-2%
Distilled water (?????) 1000ml pH 7.0
???÷?Χ?????????????????

34. Penssay Broth (Penssay ????)
Beef extract (????) 1.5g Yeast extract (?????) 1.5g
Tryptone (???????) 5g NaCl 3.5g
Glucose (??????) 1g K2HPO4.3H2O 4.8g
Distilled water (?????) 1000ml pH 7.2

35. Blond Agar (??????????)
Peptone (??????) 10g Glucose (??????) 2g
NaCl 5g NaH2PO4 2.5g
Sodium citrate (????????) 5g Brain infusion (????) 800ml
Pig heart infusion (?????) 200ml Sleep Blood (???) 10%
pH 7.2
[Note]??Preparation of pig brain and pig heart infusion??
??1?? Smash up 100g fresh pig heart ??without fat and blood vessel??.Add distilled water up to 1000ml;
??2?? Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;
??3?? Keep the two infusions at 50?? for 1 hr , then boil for 15 minutes;
??4?? Cool and filtrate through gauze . [?]?????????????????1??????????????????????????100??????????1000????????2?????????????????200??????????1000????????3??50?汣??1С????????15???????4??????????????????á?

36. Cellulose??decomposing Bacteria Synthetic Medium (???????????????)
NaCl 6g MgSO4.7H2O 0.1g
KH2PO4 0.5g CaCl2 0.1g
K2HPO4 2.0g Yeast extract (?????) 1.0g
(NH4)2SO4 2.0g Distilled water (?????) 1000ml
Filter paper strip: one strip (?????????) pH 7.0-7.4
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. ??б????????????????2%?????2%??????????? CMCNa ??

37. Clostridium Pasreurianum Synthetic Medium ?????????????????????????
Glucose ????????? 1% MnSO4 0.001%
KH2PO4 0.05% FeSO4 0.001%
K2HPO4 0.05% Yeast extract??????? 0.1%
MgSO4.7H2O 0.02% Peptone (??????) 0.01%
NaCl 0.001% CaCO3 0.5%
Distille water (?????) 100% pH 7.0

38. Yeast Extract-Medium Extract Agar ?? (????????????? ??)
Yeast extract ??????? 4g Mall extract ??????? 10g
Glucose ????????? 4g Agar ??????? 17g
Distilled water ?????? 1000ml pH 7.3

39. Oatemeal Agar ????????????
Oatemeal??infusion??[???????] 20g Agar ??????? 18%
Trace element solution ?????????????1ml
Distilled water up to ???????????????1000?????? 1000ml pH 7.2
[Note]??Trace element solution (?????????)??
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g Distilled water (?????) 100ml

40. Calcium Malate Agar (?????????)
Calium malate (??????) 10g K2HPO4 0.5g
NH4Cl 0.5g Glycerol (????) 10g
Agar (???) 15g Distilled water (?????) 1000ml


41. Thiobacillus Ferrooxidans Medium (??????????????????)
(NH4)2SO4 0.15g KH2PO4 0.05g
KCl 0.05g MgSO4.7H2O 0.5g
Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g
Distilled water (?????) 1000ml pH 2.0

42. Thiobacillus Thiooxidans Medium (????????????????)
(NH4)2SO4 0.3g KH2PO4 3-4g
CaCl2 (anhydrous) [???] 0.25g MgSO4.7H2O 0.5g
FeSO4.7H2O 0.001g Distilled water (?????) 1000ml
pH 3.5-4

43. Plasma Substitute Medium (???????????)
Sucrose (????) 12.5-15% Peptone (??????) 0.5%
KH2PO4 0.03% Na2HPO4.12H2O 0.14%
pH 7.0-7.2

44. Skim Milk Medium (????????????) ATCC 183
?????? 100g ?????? 1000ml
???÷?Χ?????????????????????????????

45. Pea Agar (?????)
Glucose (??????) 1% Peptone (??????) 0.5%
NaCl 0.5% Agar (???) 2.0%
Pea infusion (counted by NH2) [?????(??NH2??)] 12-14 mg?M100
pH (before sterilization) [???] 7.2-7.5
[Note]: Preparetion of pea infusion (??????????) :
Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37?? for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120?? for 30 minutes. Store at cool contition . (???????900???????5?????????У????????3?????????????pH??4.3-4.5????????30???????????????????????????????????????????37???????7-10?????????????????????20?????????????????????????????????????????????120??30????????????????á?)
The standard quality of pea infusion as following (????????????)??
Aminonitrogen????????: 160-200 mg?M100ml
Soluble phosphoruse???????: 400-600 ??g?Mml
Color?????: auratus,transparent ???????????

46. Wheat Bran Medium (???????)
Wheat bran (????) 3.6% (NH4)2HPO4 0.3%
K2HPO4 0.02% MgSO4.7H2O 0.01%
Agar (???) 2% pH 7.0
???÷?Χ????ù????ù??????ù????ù??

47. Glucose Yeast Extract Agar (???????????????)
Glucose (??????) 10g Yeast extract (?????) 10g
Water (?) 1000ml Agar (???) 15g
pH 7.2

48. MMN Medium (MMN??????)
CaCl2 0.05g MgSO4 0.15g
NaCl 0.025g FeCl3 (1%) 1.2ml
KH2PO4 0.5g Vitamib B1 (????) 100??g
(NH4)2HPO4 0.25g Wort (12 Be??) 100ml
Glucose (??????) 10-15g Citric acid (??????) 0.2g
Distilled water (?????) 900ml pH 5.5 (????)
???÷?Χ???????

49. Starch Agar (???????)
Soluble starch (?????????) 10g NaNO3 1g
MgCO3 1g K2HPO4 0.3g
NaCl 0.5g Agar (???) 20g
Distilled water (?????) 1000ml

50. 5 Be??Wort Agar (5 Be?????????)
???÷?Χ???????ù???????ù???????ù??????ù

51. 5 Be??Koji Extract Agar (5 Be??????????)
???÷?Χ??????ù?????????ù

52. ????????????????YEB??
Beef extract (??????) 5g/L Yeast extract (?????) 1g/L
Peptone (??????) 5g/L Sucrose (????) 5g/L
MgSO4.7H2O 0.4g/100ml Agar (???) 1.5g/100ml
pH 7.4
???÷?Χ:?????????

53. ?????????? (Tm)
Peptone (??????) 10g Casein (????????????) 1g
Glucose (??????) 5g Ddhro (???) 1000ml
Agar(???) 15g pH 7.0

54. YM (Yeast Malt Agar)
????? 3.0g ???? 3.0g
?????? 5.0g ??? 20.0g
????? 1000ml
55. ISP 4
????????? 10.0g K2HPO4 1.0g
MgSO4.7H2O 1.0g NaCl 1.0g
(NH4)2SO4 2.0g CaCO3 2.0g
FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g
??? 20.0g ????? 1000ml

56. Trypton Soy Agar
??????? 17.0g ???? 3.0g
?????? 2.5g ??? 20.0g
NaCl 5.0g K2HPO4 2.5g
????? 1000ml

57. NA (Nutrient Agar)
???? 3.0g ?????? 5.0g
??? 20.0g ????? 1000ml
pH 6.6-7.0

58. ???(??)????? (ATCC 1)
????? 5.0g ?????? 3.0g
???(??)?? 25.0g ??? 15.0g
????? 1000ml

59. Marine??? 2216 (ATCC 2)
?????? 5.0g ????? 1.0g
???????? 0.1g ?廯?? 0.08g
NaCl 19.45g MgCl2 8.8g
Na2SO4 3.24g CaCl2 1.8g
KCl 0.55g NaHCO3 0.16g
NH4NO3 0.0016g Na2HPO4 0.008g
????? 0.034g ???? 0.022g
?????? 0.004g ?????? 0.0024g
??? 15.0g ????? 1000ml
pH 7.4-7.8

60. ??????(ATCC 4)
???(?????) 500.0g ??????? (Tryptose) 10.0g
NaCl 5.0g ??? 15.0g
????? 1000ml pH 6.6-7.0

61. ?????γ??????? (ATCC 5)
????? 1.0g ???? 1.0g
??????? 2.0g ?????? 10.0g
FeSO4 ??? ??? 15.0g
????? 1000ml pH 7.2

62. Trypticase Soy Agar (ATCC 18)
Trypticase Soy Broth 30.0g ??? 15.0g
????? 1000ml

63. YPAD (ATCC 109)
????? 10.0g ?????? 20.0g
?????? 20.0g ??? 20.0g
????? 1000ml pH 7.0

64. SP (ATCC 432)
?????? 1.0g ???? 1.0g
?????? 1g ????????? 5g
???????? 2.5g ??? 20.0g
MgSO4.7H2O 0.5g K2HPO4 0.25g
????? 1000ml pH ???

65. ???????????????? (TSA-YE)
??????? 15.5g ??????? 5.0g
NaCl 5.0g ????? 6.5g
??? 15.0g ????? 1000ml
pH 7.3

66. ATCC 20
?????? 5.0g ????? 15.0g
K2HPO4 3.0g ?????? 2.0g
??? 20.0g ????? 1000ml
pH 7.3-7.5

67. TY (Yeast Tryptone Agar)
?????? 10.0g ????? 5.0g
?????? 1.0g NaCl 5.0g
??? 20.0g ????? 1000ml
pH 7.0

68. ??(Thiol)?????? (ATCC 49)
???????(Proteose Peptone No.3,Difco) 10.0g ????? 5.0g
?????? 1.0g NaCl 5.0g
??????? 8.0g ??? 1.0g
p-?????????? 0.05g pH 6.9-7.3
69. Czapek??Dox ????????? (ATCC 134)
Czapek??Dox Broth 35.0g ??? 15.0g
????? 1000ml
70. MG/L
?????? 5.0g ????? 5.0g
??????? 1.15g ?????? 0.0001g
K2HPO4 0.25g NaCl 0.1g
MgSO4.7H2O 0.1g ????? 2.5g
??? 20.0g ????? 1000ml
??ù??(?????) 50??g/ml
pH 7.0

71. Eclb Broth
?????? 10.0g ????? 5.0g
NaCl 5.0g ??? 20.0g
????? 1000ml ??????ù??(?????) 300??g/ml
pH 7.0

72. Bennetts Agar (IFO 228)
????? 1.0g ???? 1.0g
NZ Amine,type A 2.0g ?????? 10.0g
??? 20.0g ????? 1000ml
pH 7.3

73. Harrold%u2cas M40Y (ATCC 319)
????? 20.0g ????? 5.0g
???? 400.0g ??? 20.0g
????? 1000ml

74. PSA (Potato Sucrose Agar)
???? 200g ???? 20g
????? 1000ml ??? 20g
pH 6.0-6.5
75. ??????
???? 300g ???????? 17.5g
????????? 1.5g ??? 20.0g
????? 1000ml ?????? (?????) 100??g/ml
??????ù?? (?????) 100??g/ml pH 7.3-7.4

76. ????????????????
????? 20.0g ???? 5.0g
?????? 3.0g ?????? 200.0g
pH 7.0-7.2

77. ISP 2
????? 4.0g ???? 10.0g
?????? 4.0g ??? 20.0g
????? 1000ml pH 7.0

78. BHI (Brain Heart Infusion)
??? 200.0g ??????? 250.0g
?????? 10.0g ?????? 2.0g
NaCl 5.0g ??? 20.0g
????? 1000ml pH 6.8-7.2

79. YPG ??Yeast Peptone Glucose??
????? 10.0g ?????? 20.0g
?????? 20.0g ??? 20.0g
????? 1000ml pH 7.0

80. ??????????????ATCC 340??
???????????? 25.0g ??? 20.0g
????? 1000ml
*??????????????1/2С??????????????????????????С?

81. ???????(ATCC 423)
200?????????г?С????1000ml??????????1С?,??????????.???5.0mgMnSO4,pH????6.8,?????????????1000ml?????15.0g????????????????????

82. ????????????????????(ATCC 470)
???? 2000g MnSO4.5H2O 0.02g
MgSO4.7H2O 0.4g ZnSO4.7H2O 0.01g
CuSO4. 5H2O 0.01g FeSO4.7H2O 0.1g
CaCl2.2H2O 0.1g K2HPO4 0.5g
* ???200g?????????,????1000ml????????30????,??????????,????м?????????,????????1000ml??

83. ????ε??????????????ATCC 527??
????????? 10.0g K2HPO4 1.0g
MgSO4 1.0g NaCl 1.0g
??NH4??2SO4 2.0g CaCO3 2.0g
FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g
ZnSO4.7H2O 0.001g ??? 20.0g
pH 7.0-7.4

84. (AS125)
??????? 15-16 ????? 375ml
2%???(?)?? 10.0g K4HPO4 1.5g
MgSO4.7H2O 0.375g ?????? 2.25g
???? 7.5ml ?????? 18.8g

85. ?????(Oatmeal)?????????
????? 60.0g ??? 12.5g
????? 1000ml
* ?????????600ml??У???????????????45-50oC????????????????????400ml???121oC???90?????

86. ???????????ATCC 573??
??NH4??2SO4 1.3g K2HPO4 0.37g
MgSO4.7H2O 0.25g CaCl2.2H2O 0.07g
FeCl3 0.02g ?????? 1.0g
????? 1.0g ????? 1000ml
??10N H2SO4 ??pH??4.0????????????????????????????????????pH?????3.5?????????????40g/L?????????????????????50oC????????????????????????????????????????????????

87. ????????????ATCC 593??
????????? 500.0g ????? 1000ml
NaOH 25.0ml
* ??????????????????????????????Χ??????????????NaOH?????????????????????????????????????????????????????????????????1000ml??????м????г???
?????? 30.0g ????? 5.0g
K2HPO4 5.0g 0.025%??????Resazurin?????4.0ml
?????У????????0.5g L-???????HCl??H2O??pH????7.0????????????????O2-freenitrogen????3%?????????????1???????4-5???????????????????????????????????????????????????????????????????15?????

88. YMPG
????? 3.0g ???? 3.0g
?????? 5.0g ?????? 10.0g
FeSO4.7H2O 0.1g ??? 20.0g
????? 1000ml pH 6.8
?????110oC??35????

89. TGYM??????(ATCC 679)
??????? 5.0g ?????? 1.0g
????? 3.0g ????? 1000ml
DL-??????(DL-Meth 0.5g
90. TYG??????(ATCC 741)
??????? 3.0g ????? 3.0g
?????? 3.0g K2HPO4 1.0g
??? 20.0g ????? 1000ml
pH 7.4


91. M9
Na2HPO4 12.8g KH2PO4 3.0g
NaCl 0.5g NH4Cl 0.5g
??????(???????) 10.0g ?????B1(???????) 1??g/ml
pH ???

92. ISP3 (IFO 245)
????? 20.0g ????????? 1ml
pH 7.2
???????
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g

93. Oatmeal Agar
??? 5.0g ????? 500ml
???????????????? 40.0g ????? 250ml
??????????????????????1000ml pH 5.8

94. V8-Juice Agar (AYCC 343)
V-8 ??? 200g CaCO3 3.0g
??? 20.0g ????? 1000ml
pH 5.8

95. Czapek-Dox Agar (ATCC 312)
NaNO3 3.0g K2HPO4 1.0g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.7H2O 0.01g ????(???????) 30.0g
??? 20.0g ????? 1000ml
pH 5.6

96. GC?????? (ATCC 814)
A ?
??? 5.0g GC?????????(BBL 11275) 36.0g
????? 500.0ml
B ?
???????????(BBL 11871) 10.0g ????? 500.0ml
Iso Vitale X (BBL 11876) 10.0ml

97. ???????????? (ATCC 838)
K2HPO4 0.1g KH2PO4 0.4g
MgSO4.7H2O 0.2g NaCl 0.1g
CaCl2 0.02g FeCl3 0.01g
NaMoO4.2H2O 0.002g ???????(Na malate) 5.0g
????? 0.05g ????? 1000ml
pH 7.2-7.4

98. BG-11
NaNO3 1.5g K2HPO4 0.04g
MgSO4.7H2O 0.075g CaCl2.7H2O 0.036g
Na2CO3 0.02g ?????? 0.006g
???????? 0.006g *????????? A5 1ml
??????ù?? (?????) 50??g/ml ????? 1000ml
??? 20.0g
* ????????? A5
H3BO4 2.86g MnCl2.4H2O 1.81g
ZnSO4 0.222g Na2MoO4 0.39g
CuSO4.5H2O 0.079g Co(NO3)2.6H2O 49.4g

99. (Marine Agar)
?????? 5.0g ????? 1.0g
???????? 0.1g NaCl 19.45g
MgSO4.7H2O 5.9g KCl 0.55g
Na2SO4 3.24g CaCl2 1.8g
NaHCO3 0.16g KI 0.08g
?????? 10ml pH 7.0-7.6
* ???????????
????? 0.034g ?????? 0.004g
????? 1000ml H3BO4 0.022g
NaCl 0.0024g NaNO3 0.0016g
NaHPO4 0.008g ????? 1000ml

100.(Yeast Starch Agar)
????? 2.0g ????????? 10.0g
??? 20.0g ????? 1000ml
pH 7.2-7.4

101.GYMC
?????? 4.0g ????? 2.0g
???? 20.0g ??? 20.0g
CaCO3 1.5g ????? 1000ml
pH 6.8-7.0

102. LCSB
???? 15.0g ????? 5.0g
?????? 5.0g NaCl 4.0g
MgSO4.7H2O 0.5g KH2PO4 0.6g
FeCl3.6H2O 0.005g CuSO4.5H2O 0.002g
??? 20.0g ????? 1000ml
?????? 65% pH 4.8

103.????1??(G)
????????? 20.0g KNO3 1.0g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4.7H2O 0.01g
??? 20.0g ????? 1000ml
pH 7.0-7.2

104.??????-????????(ATCC 846)
????? 5.0g ?????? 1.0g
KH2SO4 1.0g NaCl 1.0g
??? 20.0g ????? 950.0ml
pH 6.8
????50ml10%??????(????????????????????)??

105.Tep???(Uric Acid)???(ATCC 894)
Na2HPO4.12H2O 9.0g KH2PO4 1.5g
MgSO4.7H2O 0.2g ?????????(???) 1.2g
CaCl2 20.0mg MnCl2.4H2O 1.0mg
Trypticcase Soy Broth (BBL 11768) 3.0g ???? 4.0g
??? 20.0 ????? 1000ml
pH 7.2

106.?????????????????? (ATCC 1053)
??????? 10.0g ???? 10.0g
????? 3.0g ?????? 5.0g
NaCl 5.0g ????????? 1.0g
????????????? 0.5g ?????? 3.0g
??? 15.0g ????? 1000ml
pH 6.6-7.0

107.?????-???(??)????? (ATCC 1205)
K2HPO4 0.5g MgSO4.7H2O 0.2g
NaCl 0.1g ???(??)?? 10.0g
????? 0.4g ????????? 1000ml

108.Tomato Dextrin Agar (ATCC 965)
????? 20.0g ???? 20.0g
????? 10.0g CoCl2.6H2O 5.0mg
??? 20.0g ????? 1000ml
pH 7.2-7.4

109.????????????? (ATCC 1554)
FeSO4 1.0mg MgSO4.7H2O 200.0mg
Na2HPO4 210.0mg NaH2PO4 90.0mg
KCl 40.0mg CaCl2 15.0mg
NaNO3 0.25g NH4Cl 0.25g
?????????(??????) 10.0ml
n-Heptadecane(C17) 0.1%(v/v) ????? 1000ml
???????????
CuSO4.5H2O 500mcg H3BO4 1.0mg
MnSO4.5H2O 7.0mcg ZnSO4.7H2O 7.0mg
MoO3 1.0mg CoSO4.7H2O 18.0mcg
????? 1000ml

110.?????????? (AS 1)
?????? 100.0g ????? 10.0g
CaCO3 20.0g ??? 15.0g
????? 1000ml pH 6.8
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爱一个人，比恨一个人难的太多了
2楼2012-04-23 12:33:03
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daiybe

金虫 (小有名气)

daiybe

【答案】应助回帖

上述资料，楼主好好寻找一下
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爱一个人，比恨一个人难的太多了
3楼2012-04-23 12:43:33
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wgy2007

木虫 (小有名气)

【答案】应助回帖

★ ★ ★ ★ ★
感谢参与，应助指数 +1
hanbin7686: 金币+5, ★★★很有帮助 2012-04-25 11:01:18
用MRS培养基
1. 将以下成分溶解在850毫升的蒸馏水中
- 酪蛋白胨                 10g
- 牛肉膏                10g
- 酵母膏                 5g
- K2HPO4•3H2O         2g   
- 乙酸钠                  5g
- 葡萄糖                20g
- 吐温80                 1ml
- 柠檬酸二铵         2g
- MgSO4•7H2O         0.58g
- MnSO4•4H2O         0.25g
2. 调节pH到6.2 – 6.5
最后定容到1000ml
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4楼2012-04-24 11:01:43
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hanbin7686

银虫 (初入文坛)
谢谢您了  呵呵  一会就配置下试试了  呵呵
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5楼2012-04-25 11:00:59
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busiyu1437

银虫 (初入文坛)
M17G
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6楼2012-04-25 21:39:41
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erlangshen89

木虫 (小有名气)
你好，你在做乳酸链球菌吗？可否详细咨询一下您？
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7楼2012-12-04 15:24:21
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