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[求助]
含有dehybridize的生物分析过程求解析
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这个是文献中的DNA分析内容（图附后）： DNA Assay Based on Magnetic Microbeads. Excess 3′-terminal biotinylated capture oligo was incubated with 1 mL of 1B&W buffer (pH)7.5, 1.0 mol/L NaCl, 0.5 mmol/L EDTA, 5 mmol/L tris-HCl) of washed 200 íg of streptavidin-coated magnetic microbeads for 15 min. After magnetic separation and washing to remove unbound oligonucleotide, the microbeads were resuspended in 1 mL of 1hybridization buffer (pH)7.0, 0.75 mol/L NaCl, 10 mmol/L sodium citrate). Excess NPs labeled probe oligo and different amounts of the target oligo were added to the above microbeads solution and slow-tilt rotated for 1 h. The microbeads were separated magnetically and washed three times with 1hybridization buffer. Then 1 mL of 0.1 mol/L fresh NaOH solution was added to the microbeads precipitate and stirred for 3 min, and the supernatant was removed in the magnetic stand for the time-resolved fluorescence assay.  图.png |
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2楼2013-04-10 01:17:34
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