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[ÇóÖú] º¬ÓÐdehybridizeµÄÉúÎï·ÖÎö¹ý³ÌÇó½âÎö

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DNA Assay Based on Magnetic Microbeads.
Excess 3¡ä-terminal biotinylated capture oligo was incubated with 1 mL of
1B&W buffer (pH)7.5, 1.0 mol/L NaCl, 0.5 mmol/L EDTA,
5 mmol/L tris-HCl) of washed 200 ¨ªg of streptavidin-coated
magnetic microbeads for 15 min. After magnetic separation and
washing to remove unbound oligonucleotide, the microbeads were
resuspended in 1 mL of 1hybridization buffer (pH)7.0, 0.75
mol/L NaCl, 10 mmol/L sodium citrate). Excess NPs labeled
probe oligo and different amounts of the target oligo were added
to the above microbeads solution and slow-tilt rotated for 1 h. The
microbeads were separated magnetically and washed three times
with 1hybridization buffer. Then 1 mL of 0.1 mol/L fresh NaOH
solution was added to the microbeads precipitate and stirred for
3 min, and the supernatant was removed in the magnetic stand
for the time-resolved fluorescence assay.

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