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hjn2008

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虫号: 2071006
注册: 2012-10-18
专业: 光谱分析

[求助] 含有dehybridize的生物分析过程求解析

这个是文献中的DNA分析内容（图附后）：
DNA Assay Based on Magnetic Microbeads.
Excess 3′-terminal biotinylated capture oligo was incubated with 1 mL of
1B&W buffer (pH)7.5, 1.0 mol/L NaCl, 0.5 mmol/L EDTA,
5 mmol/L tris-HCl) of washed 200 íg of streptavidin-coated
magnetic microbeads for 15 min. After magnetic separation and
washing to remove unbound oligonucleotide, the microbeads were
resuspended in 1 mL of 1hybridization buffer (pH)7.0, 0.75
mol/L NaCl, 10 mmol/L sodium citrate). Excess NPs labeled
probe oligo and different amounts of the target oligo were added
to the above microbeads solution and slow-tilt rotated for 1 h. The
microbeads were separated magnetically and washed three times
with 1hybridization buffer. Then 1 mL of 0.1 mol/L fresh NaOH
solution was added to the microbeads precipitate and stirred for
3 min, and the supernatant was removed in the magnetic stand
for the time-resolved fluorescence assay.

图.png

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