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3006213050

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2Â¥: Originally posted by sally208 at 2012-11-16 00:51:33
Since semiconductor QDs possess a wide excitation range, thus as soon as you can have QD with emission above 600 nm, they can serve your purpose 'Á¿×ÓµãÊÇÔÚ500¼¤·¢£¬·¢Éä·åÔÚ600+µÄλÖÃ'.
So you have  ...

Thank you for your useful reference. As we know, the emission of QDs can be near- infrared. However, the excitiation range usually was 400-480 nm. such as your ref.2. Making the 500 nm excitaion was a big problem for me. I wish you can give me some advices.
                                                              Sincerely yours.
4Â¥2012-11-16 09:06:51
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sally208

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3006213050: ½ð±Ò+10, ¡ï¡ï¡ïºÜÓаïÖú 2012-11-16 09:10:40
Since semiconductor QDs possess a wide excitation range, thus as soon as you can have QD with emission above 600 nm, they can serve your purpose 'Á¿×ÓµãÊÇÔÚ500¼¤·¢£¬·¢Éä·åÔÚ600+µÄλÖÃ'.
So you have many choices, such as CdTe, CdSe QDs, you just need to control their core size. Few examples in the literature: http://pubs.rsc.org/en/Content/ArticleLanding/2012/NR/c2nr31809a ; http://pubs.rsc.org/en/Content/ArticleLanding/2011/NR/c1nr10933b ; http://pubs.rsc.org/en/Content/ArticleLanding/2011/NR/c1nr10218d ...

Commercially available Qds from Invitrogen: http://www.invitrogen.com/search ... p;resultsPerPage=15

2Â¥2012-11-16 00:51:33
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3006213050: ½ð±Ò+5, ¡ï¡ï¡ïºÜÓаïÖú 2012-11-16 09:10:46
Á¿×ÓµãµÄ¼¤·¢¹âÆ×ºÜ¿í£¬Ö»Òª·¢ÉäÊÇÔÚ600nm¾Í¿ÉÒÔÁË¡£¿ÉÓÃCdSe/ZnS
3Â¥2012-11-16 08:48:34
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sally208

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4Â¥: Originally posted by 3006213050 at 2012-11-16 02:06:51
Thank you for your useful reference. As we know, the emission of QDs can be near- infrared. However, the excitiation range usually was 400-480 nm. such as your ref.2. Making the 500 nm excitaion was ...

I think it is just a matter of excitation efficiency at different wavelength. It is normal that he more bule the excitation wavelength you choose, the lower brightness will the QDs be. Once you have QDs with large quantum yield (e.g., QY > 20%), I do not think it can be a big issue for your experiment, even in single particle imaging experiment with the common lasers, 488 nm or even 532 nm.
5Â¥2012-11-16 15:21:40
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