| 查看: 426 | 回复: 2 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
real339金虫 (正式写手)
|
[求助]
求可编辑的PBI121和PC1301两个质粒的图谱。
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| 如图，最好是gb格式的，或者能在DNAman,Vector NT 等软件直接编辑的。 |
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【答案】应助回帖
| p1301-GATCTGAGGGTAAATTTCTAGTTTTTCTCCTTCATTTTCTTGGTTAGGACCCTTTTCTCTTTTTATTTTTTTGAGCTTTGATCTTTCTTTAAACTGATCTATTTTTTAATTGATTGGTTATGGTGTAAATATTACATAGCTTTAACTGATAATCTGATTACTTTATTTCGTGTGTCTATGATGATGATGATAGTTACAGAACCGACGACTCGTCCGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAACTGTGGAATTGATCAGCGTTGGTGGGAAAGCGCGTTACAAGAAAGCCGGGCAATTGCTGTGCCAGGCAGTTTTAACGATCAGTTCGCCGATGCAGATATTCGTAATTATGCGGGCAACGTCTGGTATCAGCGCGAAGTCTTTATACCGAAAGGTTGGGCAGGCCAGCGTATCGTGCTGCGTTTCGATGCGGTCACTCATTACGGCAAAGTGTGGGTCAATAATCAGGAAGTGATGGAGCATCAGGGCGGCTATACGCCATTTGAAGCCGATGTCACGCCGTATGTTATTGCCGGGAAAAGTGTACGTATCACCGTTTGTGTGAACAACGAACTGAACTGGCAGACTATCCCGCCGGGAATGGTGATTACCGACGAAAACGGCAAGAAAAAGCAGTCTTACTTCCATGATTTCTTTAACTATGCCGGAATCCATCGCAGCGTAATGCTCTACACCACGCCGAACACCTGGGTGGACGATATCACCGTGGTGACGCATGTCGCGCAAGACTGTAACCACGCGTCTGTTGACTGGCAGGTGGTGGCCAATGGTGATGTCAGCGTTGAACTGCGTGATGCGGATCAACAGGTGGTTGCAACTGGACAAGGCACTAGCGGGACTTTGCAAGTGGTGAATCCGCACCTCTGGCAACCGGGTGAAGGTTATCTCTATGAACTCGAAGTCACAGCCAAAAGCCAGACAGAGTCTGATATCTACCCGCTTCGCGTCGGCATCCGGTCAGTGGCAGTGAAGGGCCAACAGTTCCTGATTAACCACAAACCGTTCTACTTTACTGGCTTTGGTCGTCATGAAGATGCGGACTTACGTGGCAAAGGATTCGATAACGTGCTGATGGTGCACGACCACGCATTAATGGACTGGATTGGGGCCAACTCCTACCGTACCTCGCATTACCCTTACGCTGAAGAGATGCTCGACTGGGCAGATGAACATGGCATCGTGGTGATTGATGAAACTGCTGCTGTCGGCTTTCAGCTGTCTTTAGGCATTGGTTTCGAAGCGGGCAACAAGCCGAAAGAACTGTACAGCGAAGAGGCAGTCAACGGGGAAACTCAGCAAGCGCACTTACAGGCGATTAAAGAGCTGATAGCGCGTGACAAAAACCACCCAAGCGTGGTGATGTGGAGTATTGCCAACGAACCGGATACCCGTCCGCAAGGTGCACGGGAATATTTCGCGCCACTGGCGGAAGCAACGCGTAAACTCGACCCGACGCGTCCGATCACCTGCGTCAATGTAATGTTCTGCGACGCTCACACCGATACCATCAGCGATCTCTTTGATGTGCTGTGCCTGAACCGTTATTACGGATGGTATGTCCAAAGCGGCGATTTGGAAACGGCAGAGAAGGTACTGGAAAAAGAACTTCTGGCCTGGCAGGAGAAACTGCATCAGCCGATTATCATCACCGAATACGGCGTGGATACGTTAGCCGGGCTGCACTCAATGTACACCGACATGTGGAGTGAAGAGTATCAGTGTGCATGGCTGGATATGTATCACCGCGTCTTTGATCGCGTCAGCGCCGTCGTCGGTGAACAGGTATGGAATTTCGCCGATTTTGCGACCTCGCAAGGCATATTGCGCGTTGGCGGTAACAAGAAAGGGATCTTCACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAAGCTAGCCACCACCACCACCACCACGTGTGAATTACAGGTGACCAGCTCGAATTTCCCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGGGAATTAAACTATCAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAAAGAGCGTTTATTAGAATAACGGATATTTAAAAGGGCGTGAAAAGGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAACCACAGGGTTCCCCTCGGGATCAAAGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACGTTCAGTGCAGCCGTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTACGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTCGCGTGTTTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGCCATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCACCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGCTGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCCGGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAGTGACCAGGCTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATTGCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGTAGCCTGGCAGAGCCGTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTTCGCCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGCGGGCGCGAGGCCGCCAAGGCCCGAGGCGTGAAGTTTGGCCCCCGCCCTACCCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGATCGACCAGGAAGGCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACCCTGTACCGCGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAGGCGGCGCGGTGCCTTCCGTGAGGACGCATTGACCGAGGCCGACGCCCTGGCGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCAGGACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATGATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGTGCGGCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGCCGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGATGTGTATTTGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGATGCGATGAGTAAATAAACAAATACGCAAGGGGAACGCATGAAGGTTATCGCTGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCATCTAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCCGATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGATCAACCGCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGGCCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCGGACTTGGCTGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGTGCAGCCAAGCCCTTACGACATATGGGCCACCGCCGACCTGGTGGAGCTGGTTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTGTCGTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGCGCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACGCAGCGCGTGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAACCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAAATCAAAACTCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCACAAACACGCTAAGTGCCGGCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAACGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTCAGTTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGGCAAGACCATTACCGAGCTGCTATCTGAATACATCGCGCAGCTACCAGAGTAAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGCGGCTAAAGGAGGCGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCATGTGTGGAGGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGTCTGCCGGCCCTGCAATGGCACTGGAACCCCCAAGCCCGAGGAATCGGCGTGACGGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGATGACCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCGAGGCAGAAGCACGCCCCGGTGAATCGTGGCAAGCGGCCGCTGATCGAATCCGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCCGTCGATTAGGAAGCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATGACGTGGGCACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGTCTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTACGAGCTTCCAGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTGTGGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATGAACCGATACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCCACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGCAGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTTGCCATGCAGCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATCCGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAAGAGCGAAACCGGGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCGAGATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTTTTTGATCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCGCCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGCAGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCTGATCGGGTCAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGCAGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCTGATCGAGGGCGAAGCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCCCTAGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACATTGGGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAACCCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGTGACTGATATAAAAGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAACTCTTAAAACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCCGAAGAGCTGCAAAAAGCGCCTACCCTTCGGTCGCTGCGCTCCCTACGCCCCGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGCTGGCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCACTCGACCGCCGGCGCCCACATCAAGGCACCCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGCATTCTAGGTACTAAAACAATTCATCCAGTAAAATATAATATTTTATTTTCTCCCAATCAGGCTTGATCCCCAGTAAGTCAAAAAATAGCTCGACATACTGTTCTTCCCCGATATCCTCCCTGATCGACCGGACGCAGAAGGCAATGTCATACCACTTGTCCGCCCTGCCGCTTCTCCCAAGATCAATAAAGCCACTTACTTTGCCATCTTTCACAAAGATGTTGCTGTCTCCCAGGTCGCCGTGGGAAAAGACAAGTTCCTCTTCGGGCTTTTCCGTCTTTAAAAAATCATACAGCTCGCGCGGATCTTTAAATGGAGTGTCTTCTTCCCAGTTTTCGCAATCCACATCGGCCAGATCGTTATTCAGTAAGTAATCCAATTCGGCTAAGCGGCTGTCTAAGCTATTCGTATAGGGACAATCCGATATGTCGATGGAGTGAAAGAGCCTGATGCACTCCGCATACAGCTCGATAATCTTTTCAGGGCTTTGTTCATCTTCATACTCTTCCGAGCAAAGGACGCCATCGGCCTCACTCATGAGCAGATTGCTCCAGCCATCATGCCGTTCAAAGTGCAGGACCTTTGGAACAGGCAGCTTTCCTTCCAGCCATAGCATCATGTCCTTTTCCCGTTCCACATCATAGGTGGTCCCTTTATACCGGCTGTCCGTCATTTTTAAATATAGGTTTTCATTTTCTCCCACCAGCTTATATACCTTAGCAGGAGACATTCCTTCCGTATCTTTTACGCAGCGGTATTTTTCGATCAGTTTTTTCAATTCCGGTGATATTCTCATTTTAGCCATTTATTATTTCCTTCCTCTTTTCTACAGTATTTAAAGATACCCCAAGAAGCTAATTATAACAAGACGAACTCCAATTCACTGTTCCTTGCATTCTAAAACCTTAAATACCAGAAAACAGCTTTTTCAAAGTTGTTTTCAAAGTTGGCGTATAACATAGTATCGACGGAGCCGATTTTGAAACCGCGGTGATCACAGGCAGCAACGCTCTGTCATCGTTACAATCAACATGCTACCCTCCGCGAGATCATCCGTGTTTCAAACCCGGCAGCTTAGTTGCCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACAACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAGTGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGGGGGATCTGGATTTTAGTACTGGATTTTGGTTTTAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACATACTAAGGGTTTCTTATATGCTCAACACATGAGCGAAACCCTATAGGAACCCTAATTCCCTTATCTGGGAACTACTCACACATTATTATGGAGAAACTCGAGCTTGTCGATCGACAGATCCGGTCGGCATCTACTCTATTTCTTTGCCCTCGGACGAGTGCTGGGGCGTCGGTTTCCACTATCGGCGAGTACTTCTACACAGCCATCGGTCCAGACGGCCGCGCTTCTGCGGGCGATTTGTGTACGCCCGACAGTCCCGGCTCCGGATCGGACGATTGCGTCGCATCGACCCTGCGCCCAAGCTGCATCATCGAAATTGCCGTCAACCAAGCTCTGATAGAGTTGGTCAAGACCAATGCGGAGCATATACGCCCGGAGTCGTGGCGATCCTGCAAGCTCCGGATGCCTCCGCTCGAAGTAGCGCGTCTGCTGCTCCATACAAGCCAACCACGGCCTCCAGAAGAAGATGTTGGCGACCTCGTATTGGGAATCCCCGAACATCGCCTCGCTCCAGTCAATGACCGCTGTTATGCGGCCATTGTCCGTCAGGACATTGTTGGAGCCGAAATCCGCGTGCACGAGGTGCCGGACTTCGGGGCAGTCCTCGGCCCAAAGCATCAGCTCATCGAGAGCCTGCGCGACGGACGCACTGACGGTGTCGTCCATCACAGTTTGCCAGTGATACACATGGGGATCAGCAATCGCGCATATGAAATCACGCCATGTAGTGTATTGACCGATTCCTTGCGGTCCGAATGGGCCGAACCCGCTCGTCTGGCTAAGATCGGCCGCAGCGATCGCATCCATAGCCTCCGCGACCGGTTGTAGAACAGCGGGCAGTTCGGTTTCAGGCAGGTCTTGCAACGTGACACCCTGTGCACGGCGGGAGATGCAATAGGTCAGGCTCTCGCTAAACTCCCCAATGTCAAGCACTTCCGGAATCGGGAGCGCGGCCGATGCAAAGTGCCGATAAACATAACGATCTTTGTAGAAACCATCGGCGCAGCTATTTACCCGCAGGACATATCCACGCCCTCCTACATCGAAGCTGAAAGCACGAGATTCTTCGCCCTCCGAGAGCTGCATCAGGTCGGAGACGCTGTCGAACTTTTCGATCAGAAACTTCTCGACAGACGTCGCGGTGAGTTCAGGCTTTTTCATATCTCATTGCCCCCCGGGATCTGCGAAAGCTCGAGAGAGATAGATTTGTAGAGAGAGACTGGTGATTTCAGCGTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGATATTACCCTTTGTTGAAAAGTCTCAATAGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAGACGAGAGTGTCGTGCTCCACCATGTTATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGATATTACCCTTTGTTGAAAAGTCTCAATAGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAGACGAGAGTGTCGTGCTCCACCATGTTGGCAAGCTGCTCTAGCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGCTAGAGCAGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAGCTTCATGGAGTCAAAGATTCAAATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACAGTTCATACAGAGTCTCTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACACTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGAACACGGGGGACTCTTGACCATGGTA 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3楼2012-10-11 16:27:05
【答案】应助回帖
★ ★ ★ ★
看天: 金币+4, 好吧 鼓励一下 也是新人 2012-10-12 14:42:02
看天: 金币+4, 好吧 鼓励一下 也是新人 2012-10-12 14:42:02
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Binary vector pBI121, complete sequence GenBank: AF485783.1 ORIGIN 1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc 61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc 121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat 181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc 241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag 301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc 361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc 421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg 481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc 541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata 601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg 661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata 721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta 781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg 1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga 1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg 1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac 1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc 1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg 1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc 1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc 1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg 1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg 1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg 1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg 1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc 1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa 1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg 2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa 2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc 2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc 2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg 2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc 2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct 2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc 2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc 2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc 2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc 2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa 2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg 2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga 3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat 3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg 3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg 3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc 4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc 4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg 4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata 4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc 4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg 4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag 4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta 4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca 4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt 4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg 4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt 4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc 4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag agaacacg35S启动子 35S F:5' AGATTAGCCTTTTCAATTTCAGAAAG 3' 35S R:5' CGTGTTCTCTCCAAATGAAATGAAC 3' 35smethy F:5' GGGCAATTGAGACTTTTCAACAAAG 3' gg ggactctaga 5821 ggatccccgg gtggtcagtc ccttatgtta cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgcgggca acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta actatgccgg aatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg cactagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt gctgatggtg cacgaccacg cattaatgga ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca tcgtggtgat tgatgaaact gctgctgtcg gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc agtcaacggg gaaactcagc aagcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta acaagaaagg gatcttcact cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa aaccgcagca gggaggcaaa caatga GUS gusProbe F:5' TCCTGTAGAAACCCCAACCCGTGAA 3' gusProbe R:5' GCTGGCCTGCCCAACCTTTCGGTAT 3' R: cgtccaccca ggtgttcggc gtggtg 5' GAACATGGCATCGTGGTGATTGATG 3' 5' CATTGTTTGCCTCCCTGCTGCGGTT 3'785bp的探针 GUSF: 5'cca cta tcc ttc gca aga ccc ttc c3' GUSR: 5'cat cgc aag acc ggc aac agg att c3' ：Enzymes that do not cut（探针）: _________________________________________________________ AarI, AatII, AccI, Acc65I, AclI, AfeI, AflII, AflIII, AgeI, AhdI, AleI, AloI, AloI AlwNI, ApaI, ApaLI, ApoI, AscI, AseI, AsiSI, AvaI, AvrII, BaeI, BaeI, BamHI, BanI BanII, BbeI, BbsI, BbvI, BbvCI, BcgI, BcgI, BciVI, BfrBI, BglI, BglII, BlpI Bme1580I, BmgBI, BmrI, BmtI, BplI, BpmI, Bpu10I, BpuEI, BsaI, BsaAI, BsaBI, BsaHI BsaJI, BsaWI, BsaXI, BsaXI, BseMII, BseRI, BseYI, BsgI, BsiEI, BsiHKAI, BsiWI BslI, BsmAI, BsmBI, BsmFI, Bsp1286I, BspCNI, BspEI, BspHI, BspMI, BsrI, BsrBI BsrDI, BsrFI, BsrGI, BssHII, BssSI, BstAPI, BstBI, BstEII, BstF5I, BstXI, BstYI BstZ17I, Bsu36I, BtgI, BtsI, ClaI, DraI, DraIII, DrdI, EaeI, EagI, EarI, EciI Eco57I, EcoICRI, Eco57MI, EcoNI, EcoO109I, EcoRI, EcoRV, FalI, FokI, FseI, FspI FspAI, HaeII, HgaI, Hin4I, Hin4I, HincII, HindIII, HpaI, HphI, Hpy8I, HpyF10VI KasI, KpnI, MboII, MluI, MlyI, MmeI, MnlI, MscI, MslI, MspA1I, MwoI, NaeI, NarI NcoI, NdeI, NgoMIV, NheI, NlaIV, NotI, NsiI, NspI, PacI, PciI, PflMI, PleI, PmeI PmlI, PpiI, PpiI, PpuMI, PshAI, PsiI, PspOMI, PsrI, PsrI, PstI, PvuI, PvuII, RsrII SacI, SacII, SalI, SanDI, SapI, SbfI, ScaI, SexAI, SfiI, SfoI, SgrAI, SmaI, SmlI SnaBI, SpeI, SphI, SrfI, SspI, StuI, StyI, SwaI, TaqII, TaqII, TatI, TspDTI TspGWI, TspRI, Tth111I, XbaI, XcmI, XhoI, XmaI, XmnI, ZraI 第二个探针 F:5' TCCTGTAGAAACCCCAACCCGTGAA 3' Probe 2R: cgtccaccca ggtgttcggc gtggtg Enzymes that do not cut: _________________________________________________________ AarI, AatII, AccI, Acc65I, AclI, AfeI, AflII, AflIII, AgeI, AhdI, AloI, AloI AlwNI, ApaI, ApaLI, ApoI, AscI, AseI, AsiSI, AvaI, AvrII, BaeI, BaeI, BamHI, BanI BanII, BbeI, BbsI, BbvCI, BcgI, BcgI, BciVI, BfrBI, BglI, BglII, BlpI, Bme1580I BmgBI, BmrI, BmtI, BplI, BpmI, Bpu10I, BpuEI, BsaI, BsaBI, BsaHI, BsaWI, BsaXI BsaXI, BseMII, BseRI, BseYI, BsgI, BsiEI, BsiHKAI, BsiWI, BslI, BsmAI, BsmBI BsmFI, Bsp1286I, BspCNI, BspEI, BspHI, BspMI, BsrBI, BsrDI, BsrFI, BsrGI, BssHII BssSI, BstAPI, BstBI, BstEII, BstF5I, BstXI, BstYI, BstZ17I, Bsu36I, BtgI, BtsI ClaI, DraI, DraIII, DrdI, EaeI, EagI, EarI, EciI, Eco57I, EcoICRI, Eco57MI, EcoNI EcoO109I, EcoRI, EcoRV, FalI, FokI, FseI, FspI, FspAI, HaeII, HgaI, HincII HindIII, HpaI, KasI, KpnI, MboII, MluI, MlyI, MmeI, MnlI, MscI, MspA1I, NaeI, NarI NcoI, NdeI, NgoMIV, NheI, NlaIV, NotI, NsiI, NspI, PacI, PciI, PflMI, PleI, PmeI PmlI, PpiI, PpiI, PpuMI, PshAI, PsiI, PspOMI, PsrI, PsrI, PstI, PvuI, PvuII, RsrII SacI, SacII, SalI, SanDI, SapI, SbfI, ScaI, SexAI, SfiI, SfoI, SgrAI, SmaI, SmlI SpeI, SphI, SrfI, SspI, StuI, StyI, SwaI, TaqII, TatI, TspDTI, TspGWI, TspRI Tth111I, XbaI, XcmI, XhoI, XmaI, XmnI, ZraI 甲基化下游5' GCCCAACCTTTCGGTATAAAGACTT 3' Position Enzyme(s) __________________________________________________________________________ 51 HaeIII GG'CC 243 HaeIII GG'CC 623 HaeIII GG'CC 968 HaeIII GG'CC 1472 HaeIII GG'CC Enzymes that do not cut: _________________________________________________________ AatII, AccI, Acc65I, AclI, AfeI, AflII, AgeI, AhdI, AloI, AloI, ApaI, AscI, AsiSI AvaI, AvrII, BaeI, BaeI, BamHI, BanI, BanII, BbeI, BbsI, BbvCI, BfrBI, BglI, BglII BlpI, BmgBI, BmtI, BplI, BpmI, Bpu10I, BpuEI, BsaI, BsaHI, BsaXI, BsaXI, BseRI BseYI, BsiWI, BsmAI, BsmBI, BspEI, BsrBI, BsrDI, BsrFI, BssHII, BssSI, BstAPI BstEII, BstXI, BstZ17I, Bsu36I, ClaI, DraI, DraIII, DrdI, EagI, EcoICRI, EcoNI EcoO109I, EcoRI, FalI, FseI, FspI, FspAI, HindIII, HpaI, KasI, KpnI, MlyI, MmeI MspA1I, NaeI, NarI, NcoI, NdeI, NgoMIV, NheI, NotI, NsiI, PacI, PflMI, PleI, PmeI PmlI, PpiI, PpiI, PpuMI, PsiI, PspOMI, PstI, PvuI, PvuII, RsrII, SacI, SacII, SalI SanDI, SapI, SbfI, ScaI, SexAI, SfiI, SfoI, SgrAI, SmaI, SmlI, SpeI, SphI, SrfI StuI, StyI, SwaI, TaqII, Tth111I, XbaI, XcmI, XhoI, XmaI, XmnI, ZraI 单酶切 atca acaactctcc tggcgcacca 7681 tcgtcggcta cagcctcggg aattgctacc gagctcgaat ttcccc gatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcNOS终止子g 7981 ggaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 8041 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 8101 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc 8161 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 8221 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat 8281 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 8341 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc 8401 tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa caggattttc 8461 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 8521 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca gtacattaaa 8581 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc 8641 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat 8701 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact 8761 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac 8821 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg 8881 atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc atttctcgct 8941 taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa tcgctggata 9001 aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga cgatatcaac 9061 tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc gactgtcggc 9121 ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca gtaaggaaac 9181 aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc 9241 gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg 9301 atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt 9361 aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat 9421 ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt 9481 caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg 9541 tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc 9601 taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa 9661 tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat 9721 ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc 9781 gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc 9841 gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta 9901 ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc 9961 ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat 10021 ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat 10081 tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga 10141 tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg 10201 ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc 10261 aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct 10321 tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg 10381 aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt 10441 cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca 10501 gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca 10561 cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg 10621 gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa 10681 taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc 10741 ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca 10801 tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa 10861 atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca 10921 gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac 10981 aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg 11041 gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata 11101 gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga 11161 cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg 11221 tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg 11281 cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc 11341 gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct 11401 tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct 11461 ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc 11521 tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc 11581 ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag 11641 tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc 11701 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa 11761 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc 11821 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt 11881 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc 11941 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc 12001 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc 12061 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa 12121 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata 12181 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg 12241 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc 12301 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata 12361 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc 12421 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc 12481 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat 12541 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca 12601 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg 12661 aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga 12721 ggtgctccag tggcttctgt gtctatcagc tgtccctcct gttcagctac tgacggggtg 12781 gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa 12841 acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt 12901 gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc 12961 aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc 13021 cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta 13081 aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc 13141 acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct 13201 ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa 13261 agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga 13321 ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc 13381 atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta 13441 cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca agcattagtc 13501 catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg ctgcctatgc 13561 cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata tattatctta 13621 tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc ctcttcgtct 13681 tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca attctcgttt 13741 tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt atcgcacccc 13801 cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta aaaattgccg 13861 gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg ccgccaccca 13921 ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc acctgcggca 13981 cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact gccccgatcc 14041 cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg 14101 ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg 14161 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca 14221 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac 14281 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat 14341 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat 14401 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc 14461 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc 14521 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt 14581 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc 14641 gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct 14701 ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccgg |
2楼2012-10-11 16:26:01