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金虫 (小有名气)

ACI: 16
应助: 85 (初中生)
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虫号: 1607967
注册: 2012-02-09

【答案】应助回帖

★ ★ ★
soundhorizo: 金币+3 2012-08-09 12:47:30
soundhorizo: ACI+1, 应助辛苦 ^^ 2012-08-10 00:00:58

reserpine: 弱碱性物质
Column : ODS column 2.0mm I.D. x 50 mmL
Mobile phase A : 5mmol/L ammonium formate - water
Mobile phase B : acetonitrile
Gradient program : 20%B (0 min) 80%B (15 min)
Flow rate : 0.2 mL/min
Injection volume : 1 uL
Column temperature : 40 deg. C
  出峰时间大概为 12.25 min

Theobromide，碱性物质
在弱碱性条件下
Zorbax Eclipse XDB-C8 column (4.6 150 mm i.d.,
5-μm particle size) at 25°C, with a mobile phase of water–THF
(0.1% THF in water, pH 8)–acetonitrile (90:10, v/v). The flow rate
is 0.8 mL/min, and detection is by UV at 273 nm  retention time of theobromide 3.25 min

1 1-methylxanthine
2 1,3-dimethyluric acid
3 3,7-dimethylxanthine theobromide
4 1,7-dimethylxanthine
Column:  ZORBAX SB-C18 (4.6X250, 5uM, rentention time 4.9 min)
Mobile phase: A = 0.2% FA
B = AcCN w 0.2% FA
Isocratic composition:  98% A 2% B (v/v)
Flow rate: 1.5 mL/min
Injection volume: 2, 4, 6 μL, respectively
Detector: DAD, 254 nm
Flowcell: 3 μL, 2-mm flow path

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