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[求助]
请帮忙翻译一段关于生物医药方面的英文，谢谢~
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The RNA thus isolated was quantitatively analyzed and used to synthesize cDNA by RT-PCR in the presence of the reverse transcriptase SuperscriptIII (Invitrogen), with 35 dermal cycles of 94(摄氏度）for 45 sec, 55(摄氏度）for 45 sec and 72(摄氏度） for 1.5 min. Inhibitory effects on gene expression were analyzed on agarose gel. The primers used in the gene amplification were summarized in Table 1. As a control gene, GAPDH was used. [ Last edited by scswpiyly on 2012-6-14 at 16:24 ] |
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8814402
至尊木虫 (职业作家)
- 翻译EPI: 509
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3楼2012-06-14 17:10:57
8814402
至尊木虫 (职业作家)
- 翻译EPI: 509
- 应助: 18 (小学生)
- 贵宾: 0.381
- 金币: 12921.1
- 散金: 47
- 红花: 16
- 帖子: 4185
- 在线: 357.9小时
- 虫号: 1184404
- 注册: 2011-01-06
- 专业: 药物学其他科学问题
【答案】应助回帖
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scswpiyly: 金币+15, 翻译EPI+1, ★★★★★最佳答案, thanks！ 2012-06-14 17:52:58
scswpiyly: 金币+15, 翻译EPI+1, ★★★★★最佳答案, thanks！ 2012-06-14 17:52:58
| The RNA thus isolated was quantitatively analyzed and used to synthesize cDNA by RT-PCR in the presence of the reverse transcriptase Superscript III (Invitrogen), with 35 dermal cycles of 94(摄氏度）for 45 sec, 55(摄氏度）for 45 sec and 72(摄氏度） for 1.5 min. 对由此分离的RNA进行定量分析，并用于通过RT-PCR合成cDNA，在逆转录酶Superscript III 存在下，经过94摄氏度45秒、55摄氏度45秒和72摄氏度1.5分的35个循环。Inhibitory effects on gene expression were analyzed on agarose gel. The primers used in the gene amplification were summarized in Table 1. As a control gene, GAPDH was used. |
2楼2012-06-14 17:08:02