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The RNA thus isolated was quantitatively analyzed and used to synthesize cDNA by RT-PCR in the presence of the reverse transcriptase SuperscriptIII (Invitrogen), with 35 dermal cycles of 94(ÉãÊ϶ȣ©for 45 sec, 55(ÉãÊ϶ȣ©for 45 sec and 72(ÉãÊ϶ȣ© for 1.5 min. Inhibitory effects on gene expression were analyzed on agarose gel. The primers used in the gene amplification were summarized in Table 1. As a control gene, GAPDH was used. [ Last edited by scswpiyly on 2012-6-14 at 16:24 ] |
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8814402
ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 509
- Ó¦Öú: 18 (СѧÉú)
- ¹ó±ö: 0.381
- ½ð±Ò: 12916.1
- É¢½ð: 47
- ºì»¨: 16
- Ìû×Ó: 4183
- ÔÚÏß: 357.8Сʱ
- ³æºÅ: 1184404
- ×¢²á: 2011-01-06
- רҵ: Ò©ÎïѧÆäËû¿ÆÑ§ÎÊÌâ
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scswpiyly: ½ð±Ò+15, ·ÒëEPI+1, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, thanks£¡ 2012-06-14 17:52:58
scswpiyly: ½ð±Ò+15, ·ÒëEPI+1, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, thanks£¡ 2012-06-14 17:52:58
| The RNA thus isolated was quantitatively analyzed and used to synthesize cDNA by RT-PCR in the presence of the reverse transcriptase Superscript III (Invitrogen), with 35 dermal cycles of 94(ÉãÊ϶ȣ©for 45 sec, 55(ÉãÊ϶ȣ©for 45 sec and 72(ÉãÊ϶ȣ© for 1.5 min. ¶ÔÓÉ´Ë·ÖÀëµÄRNA½øÐж¨Á¿·ÖÎö£¬²¢ÓÃÓÚͨ¹ýRT-PCRºÏ³ÉcDNA£¬ÔÚÄæ×ªÂ¼Ã¸Superscript III ´æÔÚÏ£¬¾¹ý94ÉãÊ϶È45Ãë¡¢55ÉãÊ϶È45ÃëºÍ72ÉãÊ϶È1.5·ÖµÄ35¸öÑ»·¡£Inhibitory effects on gene expression were analyzed on agarose gel. The primers used in the gene amplification were summarized in Table 1. As a control gene, GAPDH was used. |
2Â¥2012-06-14 17:08:02
8814402
ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 509
- Ó¦Öú: 18 (СѧÉú)
- ¹ó±ö: 0.381
- ½ð±Ò: 12916.1
- É¢½ð: 47
- ºì»¨: 16
- Ìû×Ó: 4183
- ÔÚÏß: 357.8Сʱ
- ³æºÅ: 1184404
- ×¢²á: 2011-01-06
- רҵ: Ò©ÎïѧÆäËû¿ÆÑ§ÎÊÌâ
3Â¥2012-06-14 17:10:57













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