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The RNA thus isolated was quantitatively analyzed and used to synthesize cDNA by RT-PCR in the presence of the reverse transcriptase SuperscriptIII (Invitrogen), with 35 dermal cycles of 94(ÉãÊ϶ȣ©for 45 sec, 55(ÉãÊ϶ȣ©for 45 sec and 72(ÉãÊ϶ȣ© for 1.5 min. Inhibitory effects on gene expression were analyzed on agarose gel. The primers used in the gene amplification were summarized in Table 1.  As a control gene, GAPDH was used.

[ Last edited by scswpiyly on 2012-6-14 at 16:24 ]

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scswpiyly: ½ð±Ò+15, ·­ÒëEPI+1, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, thanks£¡ 2012-06-14 17:52:58
The RNA thus isolated was quantitatively analyzed and used to synthesize cDNA by RT-PCR in the presence of the reverse transcriptase Superscript III (Invitrogen), with 35 dermal cycles of 94(ÉãÊ϶ȣ©for 45 sec, 55(ÉãÊ϶ȣ©for 45 sec and 72(ÉãÊ϶ȣ© for 1.5 min. ¶ÔÓÉ´Ë·ÖÀëµÄRNA½øÐж¨Á¿·ÖÎö£¬²¢ÓÃÓÚͨ¹ýRT-PCRºÏ³ÉcDNA£¬ÔÚÄæ×ªÂ¼Ã¸Superscript III ´æÔÚÏ£¬¾­¹ý94ÉãÊ϶È45Ãë¡¢55ÉãÊ϶È45ÃëºÍ72ÉãÊ϶È1.5·ÖµÄ35¸öÑ­»·¡£Inhibitory effects on gene expression were analyzed on agarose gel. The primers used in the gene amplification were summarized in Table 1.  As a control gene, GAPDH was used.
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Inhibitory effects on gene expression were analyzed on agarose gel. ¶Ô»ùÒò±í´ïµÄÒÖÖÆ×÷ÓÃÔÚÇíÖ¬ÌǽºÉϼÓÒÔ·ÖÎö¡£The primers used in the gene amplification were summarized in Table 1.  »ùÒòÀ©ÔöËùÓÃÒýÎïÁÐÓÚ±í1.As a control gene, GAPDH was used.GAPDHÓÃ×÷¶ÔÕÕ»ùÒò¡£
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