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scswpiyly: 金币+15, 翻译EPI+1, ★★★★★最佳答案, thanks! 2012-06-14 17:52:58
The RNA thus isolated was quantitatively analyzed and used to synthesize cDNA by RT-PCR in the presence of the reverse transcriptase Superscript III (Invitrogen), with 35 dermal cycles of 94(摄氏度)for 45 sec, 55(摄氏度)for 45 sec and 72(摄氏度) for 1.5 min. 对由此分离的RNA进行定量分析,并用于通过RT-PCR合成cDNA,在逆转录酶Superscript III 存在下,经过94摄氏度45秒、55摄氏度45秒和72摄氏度1.5分的35个循环。Inhibitory effects on gene expression were analyzed on agarose gel. The primers used in the gene amplification were summarized in Table 1.  As a control gene, GAPDH was used.
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