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051811228

新虫 (初入文坛)

[求助] 求助如何做化合物的生物活性

求助
本人学化学的,近日合成了一些化合物,想做它的细胞毒性,查阅了些文献,看到下面一段话,但是有些地方不太懂呀,请各位大侠赐教。
Exponentially growing cells were seeded at 0.5x106per each well of a 24-well plate (Nunc) and cells were then exposed to compounds 4/6(怎样使细胞和化合物接触呀). Stock solutions of the test compounds in DMSO were used for serial dilutions in complete culture medium. Cells were exposed to the drugs for 48 h in a 5% CO2/95% air atmosphere. The
number of viable cells was counted in Bu¨rker hemocytometer using the trypan-blue exclusion assay. Dose/response curves were determined. Values of IC50 were
used as a measure of cellular sensitivity to given treatment .
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shaolei1986

铜虫 (小有名气)

【答案】应助回帖

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感谢参与,应助指数 +1
051811228: 金币+2 2012-06-06 14:33:26
这个英文描述太简单,说不清楚,就是一般的细胞培养,加药培养48小时,MTT法测IC50,多看几篇文献
有没有一种药,能让人忘掉一切不愉快的记忆
2楼2012-06-05 19:24:23
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531761955

银虫 (正式写手)

【答案】应助回帖

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051811228: 金币+2 2012-06-06 14:33:34
我们一般使用MTT法测试化合物抗肿瘤活性。于96孔板接种细胞悬液,90 μL/孔,细胞密度2×10的四次方个/mL,37℃,5 %CO2浓度的培养箱中培养12h,加10μL/孔相应浓度药物,继续培养44h, 加MTT10μL/孔培养4h, 从培养箱中取出,倒板,加DMSO100 μL/孔溶解MTT,测量570 nm波长下肿瘤细胞OD值,代入公式:(ODcontrol-ODtreated)/ ODcontrol×100 %计算抑制率
3楼2012-06-05 20:23:33
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