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051811228

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[求助] 求助如何做化合物的生物活性

求助
本人学化学的,近日合成了一些化合物,想做它的细胞毒性,查阅了些文献,看到下面一段话,但是有些地方不太懂呀,请各位大侠赐教。
Exponentially growing cells were seeded at 0.5x106per each well of a 24-well plate (Nunc) and cells were then exposed to compounds 4/6(怎样使细胞和化合物接触呀). Stock solutions of the test compounds in DMSO were used for serial dilutions in complete culture medium. Cells were exposed to the drugs for 48 h in a 5% CO2/95% air atmosphere. The
number of viable cells was counted in Bu¨rker hemocytometer using the trypan-blue exclusion assay. Dose/response curves were determined. Values of IC50 were
used as a measure of cellular sensitivity to given treatment .
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