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nnfyw木虫 (著名写手)
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[求助]
中译英求助，375字
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| 用不同浓度的正己烷或二甲苯处理菌株OS1 和菌株UD2, 发酵后测定蛋白酶产量。结果如图所示, 不同浓度有机溶剂胁迫处理均可以显著提高两株菌胞外蛋白酶产量。总的来讲，随着有机溶剂浓度的增加，蛋白酶分泌量增加，达到最大值后，增加有机溶剂浓度，蛋白酶分泌量不发生显著变化。10%的正己烷和1%的二甲苯处理OS1以及12.5%的正己烷和5%的二甲苯处理UD2，蛋白酶分泌量达到最高。有趣的是有机溶剂胁迫处理不仅可以提高野生型菌株的蛋白酶产量, 也可以提高诱变育种获得的突变菌株的蛋白酶产量。部分微生物通过增加胞蛋白酶分泌量提高其有机溶剂耐受性, 因此,有机溶剂胁迫会提高蛋白酶产量。研究者发现在发酵培养基中添加有机溶剂可以提高蛋白酶产量, 但有机溶剂可能会造成环境污染, 并且去除发酵液中的有机溶剂相对困难。用有机溶剂胁迫处理后发酵产蛋白酶, 不用在培养基中添加有机溶剂, 降低成本, 减少工艺, 绿色环保。 |
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_▕若¨|相依
木虫 (正式写手)
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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
nnfyw: 金币+9, 翻译EPI+1, ★有帮助, 有一些帮助，谢谢 2012-05-02 22:34:27
sltmac: 金币+20 2012-05-14 08:25:48
nnfyw: 金币+9, 翻译EPI+1, ★有帮助, 有一些帮助，谢谢 2012-05-02 22:34:27
sltmac: 金币+20 2012-05-14 08:25:48
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Deal with bacterial strain OS1 and UD2 with different concentration of n-hexane or xylene , measure the protease yield after fermentation. The results are shown in the figure, stress treatments with different concentration of organic solvent can all greatly improve exocellular protease yield of the two bacteria strains. In summary , the protease yield increased along with the concentration of organic solvent increasing ,but after up to the maximum, , protease yield did not change obviously when increased the concentration of organic solvent. When deal with bacterial strain OS1 with 10% of hexane and 1% of xylene and UD2 with 12.5% of hexane and 5% of xylene, protease yield reached the highest. The interesting thing is that handle stress of organic solvent can not only improve the protease yield of wild bacterial strains, but also improve the protease yield of mutated bacterial strains obained by mutagenesis breeding. Part of the microorganism secrete by increasing exocellula protease。 不专业，请指教，嘿嘿  |
4楼2012-04-24 20:20:27
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nnfyw: 金币+1, 翻译EPI+1, 能否不用软件翻译，这个明显不达标啊 2012-04-24 11:59:59
sltmac: 翻译EPI-1, EPI发放错误 2012-04-24 14:22:29
nnfyw: 金币+1, 翻译EPI+1, 能否不用软件翻译，这个明显不达标啊 2012-04-24 11:59:59
sltmac: 翻译EPI-1, EPI发放错误 2012-04-24 14:22:29
| In different concentrations are hexane or deal with OS1 and xylene strains UD2 strains after fermentation determination protease production. The results as shown in figure, different concentration organic solvent stress can greatly improve the treatments are two strains of bacteria exocellular protease production. In general, with the increasing of the concentration of organic solvent, protease secreted increased to maximum capacity, increase the concentration of organic solvent, protease secreted quantity does not have a significant change. 10% of hexane and 1% of xylene is OS1 processing and 12.5% of hexane and 5% of xylene is UD2 processing, protease secreted reached the highest amount. Interesting is organic solvent handle stress can not only improve the wild type strains protease yield, improve mutation breeding of the strains of protease mutations for production. Part of the microbial by increasing the protease secreted quantity and improve its organic solvent tolerance, therefore, organic solvent stress will improve the protease production. The researchers found that in the fermentation add organic solvent can improve the protease yield, but organic solvents may cause environmental pollution, and remove the fermented liquid organic solvent relatively difficult. Use organic solvent stress after processing ferment produce protease, need not in culture add organic solvent, lower the cost and reduce the process, green environmental protection. |
2楼2012-04-24 10:25:57
jeangong
木虫之王 (文学泰斗)
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★ ★ ★ ★ ★
sltmac: 金币-5, 违规存档, 禁止软件翻译，初犯，扣5金币 2012-04-24 14:22:13
sltmac: 金币-5, 违规存档, 禁止软件翻译，初犯，扣5金币 2012-04-24 14:22:13
| Determination of protease production, fermentation strains with different concentrations of n-hexane or xylene treatment OS1 and strains UD2. Results shown, different concentrations of organic solvent stress can be significantly increased by two bacteria extracellular protease production. Generally speaking, with increasing organic solvent concentration, the increase in protease secretion reaches a maximum value, the increase in the organic solvent concentration, and protease secretion is not a significant change. 10% hexane and 1% xylene processing OS1 and 12.5% hexane and 5% of the xylene treatment UD2, the highest protease secretion. Interestingly, the organic solvent stress can not only enhance the protease production of the wild-type strain, can also increase the mutation breeding mutant protease production. Part of the micro-organisms by increasing the amount of extracellular protease secretion increased organic solvent tolerance, therefore, the stress of organic solvents to improve protease production. The researchers found that the organic solvent added to the fermentation medium can improve the production of protease, organic solvents may cause environmental pollution, and the relatively difficult to remove the organic solvent in the fermentation broth. After the fermentation of organic solvent stress protease, not in the culture medium of organic solvents, reduce costs, reduce process green environmental. |
3楼2012-04-24 14:14:11
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5楼2012-05-02 22:48:55
