| ²é¿´: 827 | »Ø¸´: 4 | ||
| ±¾Ìû²úÉú 1 ¸ö ·ÒëEPI £¬µã»÷ÕâÀï½øÐв鿴 | ||
dingfang0707½ð³æ (ÕýʽдÊÖ)
|
[ÇóÖú]
Ũ¶ÈÔõô·Òë
|
|
|
×î½ü¿´ÁËһƪÎÄÕ£¬ÔÎÄÈçÏ£º Fatty acid methyl esters were prepared by incubation with acidified methanol. Briefly, lipids isolated by SPE or silica from TLC plates were mixed with toluene (1.0ml) by vortex mixing. Methanol containing 20 ml H2SO4/l (2.0 ml) was added, mixed briefly and incubated at 50¡æ for 18 h. The reaction mixture was cooled and neutralised with a solution (2.0 ml) containing a mixture of KHCO3 (0.25 M) and K2CO3 (0.5 M). Fatty acid methyl esters were isolated by addition of hexane (2.0 ml), separation of organic and aqueous phases by centrifugation at 1125 g for 10 min at 14¡æ and collection of the hexane layer. Samples were transferred to GC autosampler vials, dried under N2 and dissolved in dry hexane. ÇëÎÊÆäÖеÄ20 ml H2SO4/ l (2 ml) ÈçºÎÀí½â°¡£¿ |
» ²ÂÄãϲ»¶
¡¾¿¼Ñе÷¼Á¡¿»¯Ñ§×¨Òµ 281·Ö£¬Ò»Ö¾Ô¸ËÄ´¨´óѧ£¬³ÏÐÄÇóµ÷¼Á
ÒѾÓÐ5È˻ظ´
ÄÜÔ´²ÄÁÏ»¯Ñ§¿ÎÌâ×éÕÐÊÕ˶ʿÑо¿Éú8-10Ãû
ÒѾÓÐ12È˻ظ´
288Çóµ÷¼Á
ÒѾÓÐ10È˻ظ´
0703»¯Ñ§µ÷¼Á
ÒѾÓÐ4È˻ظ´
ÕÐÊÕµ÷¼Á˶ʿ
ÒѾÓÐ10È˻ظ´
271²ÄÁϹ¤³ÌÇóµ÷¼Á
ÒѾÓÐ6È˻ظ´
²ÄÁÏר˶274Ò»Ö¾Ô¸ÉÂÎ÷ʦ·¶´óѧÇóµ÷¼Á
ÒѾÓÐ8È˻ظ´
Ò»Ö¾Ô¸Î人Àí¹¤²ÄÁϹ¤³Ìר˶µ÷¼Á
ÒѾÓÐ4È˻ظ´
085601²ÄÁϹ¤³Ìר˶Çóµ÷¼Á
ÒѾÓÐ10È˻ظ´
321Çóµ÷¼Á
ÒѾÓÐ8È˻ظ´

jwdxbjzh
ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 244
- Ó¦Öú: 8 (Ó×¶ùÔ°)
- ¹ó±ö: 0.015
- ½ð±Ò: 10699.3
- É¢½ð: 437
- ºì»¨: 30
- Ìû×Ó: 3868
- ÔÚÏß: 1074.8Сʱ
- ³æºÅ: 611748
- ×¢²á: 2008-09-25
- ÐÔ±ð: GG
- רҵ: ÉúÎïÎÞ»ú»¯Ñ§

2Â¥2012-04-02 21:15:44
jwdxbjzh
ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 244
- Ó¦Öú: 8 (Ó×¶ùÔ°)
- ¹ó±ö: 0.015
- ½ð±Ò: 10699.3
- É¢½ð: 437
- ºì»¨: 30
- Ìû×Ó: 3868
- ÔÚÏß: 1074.8Сʱ
- ³æºÅ: 611748
- ×¢²á: 2008-09-25
- ÐÔ±ð: GG
- רҵ: ÉúÎïÎÞ»ú»¯Ñ§

3Â¥2012-04-02 21:39:32
jwdxbjzh
ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)
- ·ÒëEPI: 244
- Ó¦Öú: 8 (Ó×¶ùÔ°)
- ¹ó±ö: 0.015
- ½ð±Ò: 10699.3
- É¢½ð: 437
- ºì»¨: 30
- Ìû×Ó: 3868
- ÔÚÏß: 1074.8Сʱ
- ³æºÅ: 611748
- ×¢²á: 2008-09-25
- ÐÔ±ð: GG
- רҵ: ÉúÎïÎÞ»ú»¯Ñ§

4Â¥2012-04-02 21:40:16
dingfang0707
½ð³æ (ÕýʽдÊÖ)
- Ó¦Öú: 5 (Ó×¶ùÔ°)
- ½ð±Ò: 1570.1
- É¢½ð: 499
- ºì»¨: 2
- Ìû×Ó: 507
- ÔÚÏß: 208.3Сʱ
- ³æºÅ: 1177271
- ×¢²á: 2010-12-25
- ÐÔ±ð: MM
- רҵ: ϸ°ûÔöÖ³¡¢Éú³¤Óë·Ö»¯

5Â¥2012-04-02 21:42:01













»Ø¸´´ËÂ¥