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±¾Ìû²úÉú 1 ¸ö ·­ÒëEPI £¬µã»÷ÕâÀï½øÐв鿴

dingfang0707

½ð³æ (ÕýʽдÊÖ)

[ÇóÖú] Ũ¶ÈÔõô·­Òë

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Fatty acid methyl esters were prepared by incubation with
acidified methanol. Briefly, lipids isolated by SPE or silica
from TLC plates were mixed with toluene (1.0ml) by
vortex mixing. Methanol containing 20 ml H2SO4/l
(2.0 ml) was added, mixed briefly and incubated at 50¡æ
for 18 h. The reaction mixture was cooled and neutralised
with a solution (2.0 ml) containing a mixture of KHCO3
(0.25 M) and K2CO3 (0.5 M). Fatty acid methyl esters were
isolated by addition of hexane (2.0 ml), separation of
organic and aqueous phases by centrifugation at 1125 g for
10 min at 14¡æ and collection of the hexane layer. Samples
were transferred to GC autosampler vials, dried under N2
and dissolved in dry hexane.
ÇëÎÊÆäÖеÄ20 ml H2SO4/ l (2 ml) ÈçºÎÀí½â°¡£¿
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jwdxbjzh

ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)

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¡ï ¡ï ¡ï ¡ï ¡ï
dingfang0707: ½ð±Ò+2, ·­ÒëEPI+1, ¡ï¡ï¡ïºÜÓаïÖú, very useful, thanks 2012-04-02 21:35:49
dingfang0707: ½ð±Ò+3, ¡ï¡ï¡ïºÜÓаïÖú 2012-04-03 08:48:39
ÿÉýº¬20mlÁòËáµÄ¼×´¼2ml. ¼´£ºÃ¿Éý¼×´¼ÖÐÌí¼Ó20mlÁòËᣬȻºóÈ¡ÕâÖÖÈÜÒº2ml.
jwd
2Â¥2012-04-02 21:15:44
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

jwdxbjzh

ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)

ÒýÓûØÌû:
2Â¥: Originally posted by jwdxbjzh at 2012-04-02 21:15:44:
ÿÉýº¬20mlÁòËáµÄ¼×´¼2ml. ¼´£ºÃ¿Éý¼×´¼ÖÐÌí¼Ó20mlÁòËᣬȻºóÈ¡ÕâÖÖÈÜÒº2ml.

You're welcome.
jwd
3Â¥2012-04-02 21:39:32
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

jwdxbjzh

ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)

No problem.
jwd
4Â¥2012-04-02 21:40:16
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

dingfang0707

½ð³æ (ÕýʽдÊÖ)

ÒýÓûØÌû:
4Â¥: Originally posted by jwdxbjzh at 2012-04-02 21:40:16:
No problem.

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5Â¥2012-04-02 21:42:01
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