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zp198803

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10楼: Originally posted by liuanan at 2012-03-29 10:54:55:
蛋白质能溶解在ＤＭＳＯ中吗？我表示怀疑。通常有机溶剂会引起蛋白质团聚变性，异硫氰酸官能团在水溶液中就能发生反应，注意ｐＨ值。

透析之后的干燥过程是需要冷冻干燥么？还是抽真空就可以了

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11楼2012-03-31 16:53:43

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liuanan

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lixiangde: 金币+1, 感谢回帖交流 2012-03-31 23:33:50
zp198803: 金币+5, ★有帮助 2012-04-05 09:52:45

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11楼: Originally posted by zp198803 at 2012-03-31 16:53:43:
透析之后的干燥过程是需要冷冻干燥么？还是抽真空就可以了

建议冷冻干燥或者超滤管离心浓缩，超滤管的截留分子量可以选择1万左右的，选择密理博的。这两个方法比较好，常用

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12楼2012-03-31 22:49:27

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xhjggl

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lixiangde: 金币+1, 感谢回帖交流 2012-03-31 23:33:56

HOOK™ Dye Labeling Kit (FITC)
蛋白FITC标记试剂盒
Description:
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 520nm, respectively, therefore emitting green visible light.This kit utilizes Tube-O-Reactor™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort.
Features:
Dye preweighed and supplied in single use vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time.application for Labeling of a green fluorescent dye to proteins and peptides. And Suitable for antibody labeling. Store: -15～-20℃
编号包装价格产地
786-059 5 Reactions 2,709 GBios

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13楼2012-03-31 22:52:01

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