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FIG.    1. Schematic  presentation  of  the  single  specific  primer  PCR used to clone the csp gene. A 1-kb partial fragment of the csp gene was  amplified from C. sordellii NCIB10717 total DNA with KAG209 and  KAG210  PCR  primers,  designed  in  highly  conserved  regions  among Cbp,    Cnp,   and    Cpa.   Using    a  HindIII   DNA     library   of  C.   sordellii NCIB10717 as a template, PCR was conducted with mF2, located on  pKF3,  and  with  KAG212  or  KAG213,  located  on  the  known  1-kb fragment. Nucleotide sequences of the upstream, the known 1-kb, and  the  downstream  DNA  fragments  showed  an  open  reading  frame  of Csp. The thick arrowed line depicts the csp gene. The filled and open  areas indicate nucleotide sequence-determined (known) and  nondetermined (unknown) regions, respectively.
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Â¥: Originally posted by lxj341401 at 2012-03-14 10:49:05:
C.   sordellii NCIB10717 »ùÒò×éDNAÓÃHindIII øÇкóµÃµ½µÄƬ¶Î×÷Ϊģ°å¡£

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happywyh(½ð±Ò+1): лл²ÎÓë
Î÷¹Ï(½ð±Ò+1): Good£¡ 2012-03-14 22:31:10
C.   sordellii NCIB10717 »ùÒò×éDNAÓÃHindIII øÇкóµÃµ½µÄƬ¶Î×÷Ϊģ°å¡£
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latitudes

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Î÷¹Ï(½ð±Ò+2): ¹ÄÀøÐ³潻Á÷£¡ 2012-03-14 22:31:30
happywyh(½ð±Ò+1): 2012-03-15 09:27:40
Óà HindIII  Çиî C.   sordellii NCIB10717¹¹½¨µÄ DNAÎÄ¿â×÷Ϊģ°å

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Â¥: Originally posted by latitudes at 2012-03-14 11:29:55:
Óà HindIII  Çиî C.   sordellii NCIB10717¹¹½¨µÄ DNAÎÄ¿â×÷Ϊģ°å

mF2Ó¦¸ÃÊÇÖÊÁ£pKF3ÉϵÄÒ»¶ÎÐòÁУ¬ÔÚÕâ×÷PCRÉÏÓÎÒýÎïÓã¬ÅäºÏÏÂÓεÄKAG212»òKAG213ÒýÎï

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